Topoisomerase I Antikörper

Produktnummer ABIN597649

Dieses Human Polyklonal-Antikörper erkennt spezifisch Topoisomerase I in ELISA und ID. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Topoisomerase I Antikörper (ABIN597649)

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Reaktivität: Human

Wirt: Human

Klonalität: Polyklonal

Konjugat: Dieser Topoisomerase I Antikörper ist unkonjugiert

Applikation: ELISA, Immunodiffusion (ID)

Produktdetails anti-Topoisomerase I Antikörper

Spezifität: Recognizes human Scl-70 antigen. Does not recognize purified SSA, SSB, Sm, Sm/RNp and Jo-1 antigen.

Aufreinigung: Delipidated and defibrinated

Immunogen: Human Scl-70 Antigen from human plasma.

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, ID

Usage: Suitable for use in DNA conformational analysis and topology as well as DNA repair, drug resistance, cell proliferation and leukemia studies. The double-helical configuration that DNA strands naturally reside in makes them difficult to separate, and yet they must be separated if enzymes are to transcribe the sequences that encode proteins, or if chromosomes are to be replicated. In so-called circular DNA, in which double helical segment is bent around and joined in a circle, the two strands are topologically linked, or knotted. They cannot be separated by any process that does not involve the breaking of strands. Topoisomerases catalyze and guide the unknotting of DNA. Type I topoisomerases cut only one strand of DNA, type I topoisomerase of E. coli > E. coli (omega protein) relaxes negatively supercoiled DNA and does not act on positively supercoiled DNA. Type II topoisomerases cut both strands of DNA, type II topoisomerase of E. coli (DNA gyrase) increases the degree of negative supercoiling in DNA and requires ATP. It is inhibited by several antibiotics, including nalidixic acid and ovobiocin. Antibodies generated against the nuclear constituents are known as antinuclear antibodies (ANA). This includes autoantibodies directed against the extractable (soluble in physiological buffers) nuclear antigen or ENA. The most prominent of ANAs/ENAs are autoantibodies which binds to ds-DNA, ss-DNA, histones, ribonucleoproteins (RNP) and the SS-A, SS-B, Sm antigens, Jo-1, and Scl-70. Two antibodies, anti-dsDNA and anti-Sm, appear to occur only in SLE. Others occur in a variety of autoimmune and mixed connective tissue diseases. Antibody to Scl-70 antigen was originally called Scl-1. Anti-Scl-70 has been shown to react with a cellular antigen known as DNA topoisomerase I, which is responsible for the relaxation of supercoiled DNA. Anti-Scl-70 is found in ~75 % patients with the diffuse progressive form of scleroderma. The presence of anti-Scl-70 seems to exclude the presence of anti-centromere antibody, which is a marker for a subset of patients with scleroderma known as CREST (calcinosis, sclerodactyly, and telangiectasia) syndrome. The frequency of ANA-positives in various rheumatic diseases has been reported for SLE, rheumatoid arthritis (RA), progressive systemic sclerosis (PSS), polymyositis (PM), dermatomyositis (DM), mixed connective tissue diseases, drug-induced SLE, and Sjogren's syndrome (SS). Most of these studies are based on tedious fluorescent ANA (FANA). Other techniques such as RIA, immunodiffusion, hemagglutination, electrophoresis and immunoblotting are also used to define antibody specificity. Recently, immunological assays (mostly ELISA) that determine the specificity of ANA have been used in studying patients with systemic rheumatic diseases. Applications. Suitable for use in ELISA and Immunodiffusion.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile distilled water, sterile 40-50 % glycerol.

Konzentration: Lot specific

Buffer: Lyophilized from 0.01 M phosphate, 0.13 M sodium chloride,  pH 7.2, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Lyophilized powder may be stored at -20°C. Reconstituted product is stable for 1 year at -20°C.

Details zu Topoisomerase I

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Andere Bezeichnung: TOP1 / Topoisomerase I

Hintergrund: Name/Gene ID: TOP1
Family: Topoisomerase

Synonyms: TOP1, DNA topoisomerase I, DNA topoisomerase 1, TOPI, Topoisomerase (DNA) I, Topoisomerase I, Type I DNA topoisomerase

Gen-ID: 7150

UniProt: P11387

Pathways: Caspase Kaskade in der Apoptose, Stem Cell Maintenance