Cystin 1 Antikörper

Produktnummer ABIN5777007

Der Kaninchen Polyklonal anti-Cystin 1 Antikörper (ABIN5777007) detektiert spezifisch Cystin 1. Dieser Antikörper reagiert spezifisch mit Proben aus Crustacea.

Kurzübersicht für Cystin 1 Antikörper (ABIN5777007)

Target: Cystin 1 (CYS1)

Reaktivität: Crustacea

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Cystin 1 Antikörper ist unkonjugiert

Applikation: Bitte anfragen

Produktdetails anti-Cystin 1 Antikörper

Verwendungszweck: anti-CCAP, anti-Crustacean Cardioactive Peptide, rabbit, polyclonal

Aufreinigung: Insects were cooled for 15 minutes and dissections were carried out in insect saline or in solution A. Ganglia or brain were exposed by opening and pinning out the dorsal cuticle, mounted dorsal-and in same cases ventral-side up on a wax coated glas disk. Fixation Cover up the insect brain or ganglia 30 min to 120 min with one of the Solutions B. Vibratome sections Immunocytochemistry was carried out on free-floating Vibratom sections by means of the indirect immunofluorescence immunocytochemistry. Brains or ganglia were wrapped in 5% agar and cut at 20-50 μm with a Vibratome in Solution C (for the fixation with solution B1)or in Solution D (for the fixation with solution B2) at 4°C. Reduction Step (optional and only for fixation with solution B1) Vibratom sections are incubated during 10 min in the Solution C containing sodium borohydrite (0,1M) by stirring. Then, the tissue pieces are washed 5 times (15 min each) with Solution C by stirring. Sections are incubated during 12 hours at 4°C in Solution C + 30% sucrose. Washing Sections are washed 3 times (15 min each) in Solution C (for the fixation with Solution B1) and in Solution D (for the fixation with Solution B2) at room temperature. Application of antibody The final dilution of the polyclonal anti-CCAP is 1:1000 in Solution C or D (depending on the fixation, see above) + 0.25 % Triton X100 + 1% goat serum + 3% milk powder (without fat) + 0,25 % BSA. A dozen of sections can be incubated with 2ml of diluted antibody solution overnight or 48 h at 4°C by stirring. Then sections are washed 3 times 30 minutes with Solution D for both fixations by stirring. Secondary Antibody Sections are incubated with 1:600 dilution of Carbocyanin 3(Cy-3)-goat anti-rabbit complex (Jackson ImmunoResearch Laboratories, Inc.) in Solution D + 0.25 % Triton X100 + 3% milk powder (without fat) + 0,25 % BSA for 3 hours at 20°C by stirring. SOLUTIONS TO BE PREPARED Solution A cacodylate 0.1 M, sodium metabisulfite 10g/l, pH 6.2* Solution B1 (Boer-fixation) 15 ml aqueous sturated picric acid, 5 ml glutaraldehyde (25%), 0.1 ml glacial acetic acid or Solution B2 4% paraformaldehyde in Millonigs-phospate buffer (pH 7.3-7.4, 1g NaCl, 2.9 g Na2HPO4*2H2O, 0,524 g NaH2PO4*H2O and 8 g paraform-aldehyde were filled up to 200 ml with ddH2O) Solution C Tris 0.05 M (Tris (hydroxymethyl) aminomethane), sodium metabisulfite 8.5 g/l, pH 7.5* Solution D Tris 0.05 M, NaCl 8,5 g/l, pH 7.5* * Adjust pH with NaOH or HCl if necessary Tris solution can be replaced by a 0.01M phosphate solution.

Reinheit: > 97 % (HPLC)

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Supplied in 10 mM sodium phosphate pH 7.4 and glycerol

Handhabung: Please centrifuge briefly before opening (volume ≤2 ml).,avoid freeze/thaw cycles

Lagerung: -20 °C

Informationen zur Lagerung: store at -20 °C avoid freeze/thaw cycles

Haltbarkeit: 12 months

Details zu Cystin 1

Target: Cystin 1 (CYS1)

Andere Bezeichnung: CCAP

Hintergrund: The crustacean cardioactive peptide (CCAP) is a potent cardioexcitatory substance, originally identified in the pericardial organs of the shore crab, Carcinus means. It also modulates the neuronal activity in other arthropods. A CCAP-related new peptide family, the molluscan CCAP (M-CCAP) has been isolated and characterized from the snail Helix pomatia (Muneoka et al. 1994). Structural differences between the crustacean CCAP and the molluscan peptides are restricted only to the amidatedend of the molecules. NOTICE The anti-CCAP antiserum, generated against CCAP coupled to glutaraldehyde/polylysine (1:4), was tested for cross-reactivity using ELISA. No coss-reactivity was observed against 10 μg/mL of glutaraldehyde/polylysine conjugates of perisulfakinin, locustatachykinin II, FMRFamide, proctolin, adipokinetic hormone I, leucomyosuppressin, corazonin and the allatostatines, Dip-AST 2, Dip-AST 7, and Dip-AST 8. Protocol for Crustacean Cardioactive Peptide (CCAP) detection by immunocytochemistry in invertebrate nervous system

Molekulargewicht: 956.13 g/mol