L-Cysteine Antikörper

Produktnummer ABIN577698

Produktdetails

Target: L-Cysteine

Wirt: Maus

Klonalität: Monoklonal

Applikation: Immunoassay (IA), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)

Marke: AbX™

Produktmerkmale: Applications include Western blotting, Immunoassay and Immunoprecipitation Supplied in PBS with no carrier protein
For use under Non-Reducing conditions

Aufreinigung: Protein A Purified

Immunogen: Cysteine conjugated to Keyhole Limpet Hemocyanin

Klon: F2D

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Western blotting, Immunoassay, Immunohistochemistry and Immunoprecipitation
Western blotting, 1:1,000 AbX™ Anti-Cysteine Mouse Monoclonal ImmuNOhIstOChemIstry Porcine peripheral red blood cells were paraffin embedded and sectioned. 4 μm sections were de-paraffinized and rehydrated. Sections were incubated with or without additional thiol compounds and anti-cysteine anti- body for 2 hours at room temperature. Antibody concentration was 3 μg/mL. Sections were blocked pre- and post antibody treatements with 1 % BSA in PBS. Development was with a goat anti-mouse IgG antibody labeled with Alexa™ 488. RBC + Anti-Cysteine RBC + Anti-Cysteine + 20 mM Cysteine Cysteine addition was carried out at 20 mM in 1 % BSA in PBS and added with the monoclonal anti-cysteine antibody at 3 μg/mL for 1 hour at room temperature. With polymorphonuclear neutrophils addition of 20 mM cytseine reduced or eliminated the goat anti-mouse IgG-Alexa 488 signal PMN + Anti-Cysteine PMN + Anti-Cysteine + 20 mM Cysteine Nuclear staining was carried out with TOPRO at 1:500 dilution in PBS.

Kommentare:

Applications include Western blotting, Immunoassay and Immunoprecipitation Supplied in PBS with no carrier protein For use under Non-Reducing conditions

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: Phosphate Buffered Saline at pH 7.2 containing 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Lagerung: 4 °C

Informationen zur Lagerung: Short Term: 4°C. Extended: Aliquot and freeze at -20°C

Antigendetails

Target: L-Cysteine

Substanzklasse: Amino Acid

Hintergrund: Among several postranslational modifications known to date, cysteinylation has received relatively very little interest. Cysteinylation has been implicated in the regulation of immunity, protein kinase C activity and as a marker for utero-placental insufficiency as monitored by serum albumin cysteinylation. Protein S-thiolation by low molecular weight (LMW) thiols prevents the irreversible oxidation of cysteine residues during oxidative stress and plays a role in the redox regulation of thiol-containing proteins. Many Gram-positive bacteria lack glutathione and so the nature of S-thiolation in these organisms remains elusive. In the Gram-positive model organism Bacil- lus subtilis, cysteine represents the most abundant LMW thiol. One of the most obvious responses of B. subtilis to oxidative stress is the strong induction of cysteine biosynthesis genes. Although the origins of this effect are unclear, it may be a reflection of consumption of free cysteine by oxidation to cystine and the formation of mixed disulfides with proteins. The Arbor Assays AbX™ Cysteine Mouse Monoclonal is produced as a Protein A purified antibody. It will measure cysteine-protein complexes under non-reducing conditions.