Glutathione Antikörper (DyLight 488)

Produktnummer ABIN577696

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Glutathione in FACS und IF. Er zeigt eine Reaktivität gegenüber .

Kurzübersicht für Glutathione Antikörper (DyLight 488) (ABIN577696)

Target: Glutathione

Reaktivität: Bitte anfragen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Glutathione Antikörper ist konjugiert mit DyLight 488

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: L4H

Produktdetails anti-Glutathione Antikörper (DyLight 488)

Marke: AbX™

Produktmerkmale: Designed for Immunofluorescence and FACS Analysis Supplied as 50 µg in PBS For use under Non-Reducing conditions

Aufreinigung: Purified

Immunogen: Glutathione conjugated to Keyhole Limpet Hemocyanin

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: 1:5-1:50 IMMuNOFLuOresCeNCe Jurkat cells, permeabilized and incubated with our DyLight® 488 labeled Glutathione monoclo- nal, A001F-100UL, clone L4H, at a 1:25 dilution. 488 Filter unfiltered
Flow analysis of Jurkat cells processed for immunofluorescence as above. Unstained cells Stained at 1x Stained at 5x

Kommentare:

Designed for Immunofluorescence and FACS Analysis Supplied as 50 μg in PBS For use under Non-Reducing conditions

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 500 μg/mL

Buffer: 500 μg/mL in Phosphate Buffered Saline at pH 7.2 containing 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Lagerung: 4 °C

Informationen zur Lagerung: Short Term: 4°C. Extended: Aliquot and freeze at -20°C

Details zu Glutathione

Target: Glutathione

Substanzklasse: Chemical

Hintergrund: Glutathione (GSH) is the highest concentration non-protein thiol in mammalian cells and is present in concentrations of 0.5 to 10 mM. GSH plays a key role in many biological processes, including the synthesis of proteins and DNA, the transport of amino acids, and the protection of cells against oxidation. Harmful hydrogen peroxide cellular levels are minimized by the enzyme glutathione peroxidase (GP) using GSH as a reductant. The oxidized GSH dimer, GSSG, is formed from GSH and peroxide by the GP reaction. An important role of GSSG in the NFkB activating signal cascade is suggested by the fact that the potent NFkB inducer TPA increases intracellular GSSG levels and GSSG/GSH ratios. The AbX™ DyLight® 488-labeled dye is similar to Alexa 488, Cy2 or FITC. It will measure GSH-protein complexes under non-reducing conditions.