Reticulon 4 Antikörper (AA 501-515)

Produktnummer ABIN574094

Der Ziege Polyklonal Anti-Reticulon 4-Antikörper wurde für IHC und IHC (p) validiert. Er ist geeignet, Reticulon 4 in Proben von Human und Affe zu detektieren.

Kurzübersicht für Reticulon 4 Antikörper (AA 501-515) (ABIN574094)

Target: Reticulon 4 (RTN4)

Reaktivität: Human, Affe

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Reticulon 4 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Reticulon 4 Antikörper

Bindungsspezifität: AA 501-515

Marke: IHC-plus™

Spezifität: Human RTN4 / Nogo. This antibody is expected to recognize reported isoform A (NP_065393.1) and isoform E (NP_997404.1).

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Monkey (100%) Gibbon, Panda, Horse (93%) Marmoset, Dog, Bovine, Rabbit (87%) Mouse, Rat, Bat, Pig (80%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-KAQIVTEKNTSTKTS from an internal region of human RTN4 / Nogo (NP_065393.1, NP_997404.1). Percent identity by BLAST analysis: Human, Gorilla, Monkey (100%), Gibbon, Panda, Horse (93%), Marmoset, Dog, Bovine, Rabbit (87%), Mouse, Rat, Bat, Pig (80%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (2.5 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 to 5 μg/mL

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu Reticulon 4

Target: Reticulon 4 (RTN4)

Andere Bezeichnung: RTN4 / Nogo

Hintergrund: Name/Gene ID: RTN4
Family: Reticulon

Synonyms: RTN4, Human NogoA, My043 protein, Neurite outgrowth inhibitor, NOGOC, NSP-CL, Nogo protein, KIAA0886, Reticulon 5, Reticulon-4, Reticulon-5, Reticulon 4, RTN4-B1, RTN-X, RTN4-A, RTN4-B2, RTN4-C, Nbla00271, Neurite growth inhibitor 220, NI220/250, NOGO-A, Nogo-C, ASY, Foocen, Nbla10545, NOGO, Nogo-B, NSP

Gen-ID: 57142

NCBI Accession: NP_065393, NP_997404

UniProt: Q9NQC3

Pathways: Neurotrophin Signalübertragung, Regulation of Cell Size, SARS-CoV-2 Protein Interaktom