NOX5 Antikörper (Internal Region)

Produktnummer ABIN571285

Dieser Ziege Polyklonal Antikörper detektiert spezifisch NOX5 in WB, ELISA und IF. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für NOX5 Antikörper (Internal Region) (ABIN571285)

Target: NOX5 (NADPH Oxidase 5 (NOX5))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser NOX5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Güteklasse: Verified

Produktdetails anti-NOX5 Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: NOX5

Sequenz: EWHPFTISSA PEQKD

Spezifität: This antibody is expected to recognize all reported isoforms (NP_078781.3, NP_001171708.1, NP_001171709.1).

Kreuzreaktivität: Rind (Kuh), Hund, Human

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-EWHPFTISSAPEQKD

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 90 kDa band observed in Human Testis lysates (calculated MW of 86.4 kDa according to NP_078781.3). Recommended concentration: 0.5-2 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:8000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the plasma membrane and cytoplasm of HeLa and MCF7 cells. Recommended concentration:10ug/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu NOX5

Target: NOX5 (NADPH Oxidase 5 (NOX5))

Andere Bezeichnung: NOX5

Hintergrund: NOX5, NADPH oxidase, EF-hand calcium binding domain 5, MGC149776, MGC149777, NADPH oxidase 5, OTTHUMP00000164443, OTTHUMP00000164444

Gen-ID: 79400

NCBI Accession: NP_078781, NP_001171708, NP_001171709

Pathways: Proton Transport