Dystroglycan Antikörper (Internal Region)

Produktnummer ABIN571179

Dieses Anti-Dystroglycan-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von Dystroglycan in WB, ELISA, IHC, IF und FACS. Geeignet für Human.

Kurzübersicht für Dystroglycan Antikörper (Internal Region) (ABIN571179)

Target: Dystroglycan (DAG1) (Dystroglycan 1 (DAG1))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Dystroglycan Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-Dystroglycan Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: DAG1

Sequenz: HVGKHEYFMH ATDK

Spezifität: This antibody is expected to recognize both the precursor and the mature alpha-dystroglycan, but not the mature beta-dystroglycan.

Kreuzreaktivität: Human

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-HVGKHEYFMHATDK

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Human Heart. Recommended concentration: 5 μg/mL.

Western Blot: Approx 100 kDa band observed in Human Skeletal Muscle lysates (calculated MW of 97.4 kDa according to Human NP_004384.4 ). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:128000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the ER and cytoplasm of HeLa and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentr

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu Dystroglycan

Target: Dystroglycan (DAG1) (Dystroglycan 1 (DAG1))

Andere Bezeichnung: DAG1

Hintergrund: DAG1, dystroglycan 1 (dystrophin-associated glycoprotein 1), 156DAG, A3a, AGRNR, DAG, alpha-dystroglycan, beta-dystroglycan, dystroglycan 1

Gen-ID: 1605

NCBI Accession: NP_004384

Pathways: Maintenance of Protein Location, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus