POU3F3 Antikörper (Internal Region)

Produktnummer ABIN571161

Dieses Anti-POU3F3-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von POU3F3 in WB, ELISA, FACS und IF. Geeignet für Maus.

Kurzübersicht für POU3F3 Antikörper (Internal Region) (ABIN571161)

Target: POU3F3 (POU Class 3 Homeobox 3 (POU3F3))

Reaktivität: Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser POU3F3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Güteklasse: Verified

Produktdetails anti-POU3F3 Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: POU3F3 / BRN1 / OCT8

Sequenz: HMLSHAHQWV TAL

Kreuzreaktivität: Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-HMLSHAHQWVTAL, from the internal region of the protein sequence according to NP_006227.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 48 kDa band observed in Mouse Spinal Cord lysates and approx. 50 kDa in Mouse Brain lysates (calculated MW of 50.3 kDa according to Human NP_006227.1 and 50.2 kDa according to Mouse NP_032926.2). Recommended concentration: 1-3 μg/mL. Pri

Peptide ELISA: antibody detection limit dilution 1:4000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the nuclei of Neuro-2a cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of Neuro-2a cells. Recommended concentration: 10ug

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu POU3F3

Target: POU3F3 (POU Class 3 Homeobox 3 (POU3F3))

Andere Bezeichnung: POU3F3

Hintergrund: POU3F3, POU class 3 homeobox 3, BRN1, OTF8, OTF-8, POU domain, class 3, transcription factor 3, brain-1, brain-specific homeobox/POU domain protein 1, brn-1, oct-8, octamer-binding protein 8, octamer-binding transcription factor 8

Gen-ID: 5455, 18993, 192109

NCBI Accession: NP_006227