Tppp Antikörper (Internal Region)

Produktnummer ABIN571099

Dieser Ziege Polyklonal Antikörper erkennt spezifisch Tppp in WB, IHC und ELISA. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für Tppp Antikörper (Internal Region) (ABIN571099)

Target: Tppp (Tubulin Polymerization Promoting Protein (Tppp))

Reaktivität: Human, Maus, Ratte

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Tppp Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Güteklasse: Verified

Produktdetails anti-Tppp Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: TPPP/p25

Sequenz: DPSKDRAAKR LSLES

Kreuzreaktivität: Human

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-DPSKDRAAKRLSLES

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: In paraffin embedded Human Cerebellum shows strong staining of axonal bundles through the granular layer. Recommended concentration: 1-2 μg/mL. Paraffin embedded Human Cortex. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 26 kDa band observed in Human Brain (Cerebellum), Mouse and Rat Brain lysates (calculated MW of 23.7 kDa according to NP_008961.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (

Peptide ELISA: antibody detection limit dilution 1:32000.

Kommentare:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu Tppp

Target: Tppp (Tubulin Polymerization Promoting Protein (Tppp))

Andere Bezeichnung: TPPP

Hintergrund: TPPP, tubulin polymerization promoting protein, TPPP/p25, TPPP1, p24, p25, p25alpha, brain specific protein p25 alpha, glycogen synthase kinase 3 (GSK3) inhibitor p24, tubulin polymerization-promoting protein

Gen-ID: 11076, 72948, 361466

NCBI Accession: NP_008961