TGFBI Antikörper (Internal Region)

Produktnummer ABIN571098

Produktdetails anti-TGFBI Antikörper

Target: TGFBI (Transforming Growth Factor, beta-Induced, 68kDa (TGFBI))

Bindungsspezifität: Internal Region

Reaktivität: Human, Ratte

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser TGFBI Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Verwendungszweck: TGFBI

Sequenz: QLYTDRTEKL RPE

Kreuzreaktivität: Hund, Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Güteklasse: Verified

Immunogen: Peptide with sequence C-QLYTDRTEKLRPE, from the internal region of the protein sequence according to NP_000349.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: In paraffin embedded Kidney shows staining of intercalated cells of a collecting tubule. Recommended concentration: 4-6 μg/mL. This antibody has been successfully used in IHC on Rat, PMID: 26407577.

Western Blot: Approx 70 kDa band observed in Human and Rat Kidney lysates, and approx 75 kDa in Human Prostate and Human Colorectal cancer lysates (calculated MW of 74.7 kDa according to Human NP_000349.1 and 74.8 kDa according to Rat NP_446254.1). Recommend

Peptide ELISA: antibody detection limit dilution 1:32000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the golgi and plasma membrane of HeLa cells. Recommended concentration: 10μg/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu TGFBI

Target: TGFBI (Transforming Growth Factor, beta-Induced, 68kDa (TGFBI))

Andere Bezeichnung: TGFBI (TGFBI Produkte)

Hintergrund: TGFBI, transforming growth factor, beta-induced, 68 kDa, BIGH3, CDB1, CDG2, CDGG1, CSD, CSD1, CSD2, CSD3, EBMD, LCD1, RGD-containing collagen-associated protein, kerato-epithelin

Molekulargewicht: 74.7kDa

Gen-ID: 7045, 21810, 24511

NCBI Accession: NP_000349