ACAT1 Antikörper (AA 253-266)

Produktnummer ABIN571083

Dieses Anti-ACAT1-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von ACAT1 in WB, ELISA, IF und FACS. Geeignet für Human, Maus und Ratte.

Kurzübersicht für ACAT1 Antikörper (AA 253-266) (ABIN571083)

Target: ACAT1 (Acetyl-CoA Acetyltransferase 1 (ACAT1))

Reaktivität: Human, Maus, Ratte

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser ACAT1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-ACAT1 Antikörper

Bindungsspezifität: AA 253-266

Verwendungszweck: ACAT1 (aa253-266)

Sequenz: DEEYKRVDFS KVPK

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-DEEYKRVDFSKVPK, from the internal region of the protein sequence according to NP_000010.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx. 40 kDa band observed in lysates of cell lines CAC02, HEK293, HepG2, MCF7, NIH3T3 and KNRK and approx. 38-40 kDa in Human, Mouse and Rat Liver lysates (calculated MW of 45.2 kDa according to Human NP_000010.1, 44.81 kDa according to Mous

Peptide ELISA: antibody detection limit dilution 1:4000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS and A431 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration:

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu ACAT1

Target: ACAT1 (Acetyl-CoA Acetyltransferase 1 (ACAT1))

Andere Bezeichnung: ACAT1

Hintergrund: ACAT1, acetyl-Coenzyme A acetyltransferase 1, ACAT, MAT, T2, THIL, acetoacetyl Coenzyme A thiolase

Gen-ID: 38, 110446, 25014

NCBI Accession: NP_000010