EIF5A Antikörper (Internal Region)

Produktnummer ABIN571047

Dieses Anti-EIF5A-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von EIF5A in WB, ELISA und IHC. Geeignet für Human.

Kurzübersicht für EIF5A Antikörper (Internal Region) (ABIN571047)

Target: EIF5A (Eukaryotic Translation Initiation Factor 5A (EIF5A))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser EIF5A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Güteklasse: Verified

Produktdetails anti-EIF5A Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: eIF5A

Sequenz: RPPHRASFLK RLESK

Spezifität: Reported variants NP_001137233.1, NP_001961.1 and NP_001137234.1 represent identical protein:

Kreuzreaktivität: Human

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-RPPHRASFLKRLESK, from the internal region (near N Terminus) of the protein sequence according to NP_001137232.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Human Spleen, Tonsil and Vessel. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 20 kDa band observed in MOLT4 lysates (calculated MW of 20.2 kDa according to NP_001137232.1). Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour. This antibody has been successfully used in WB on Human, PMID: 27414022

Peptide ELISA: antibody detection limit dilution 1:128000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu EIF5A

Target: EIF5A (Eukaryotic Translation Initiation Factor 5A (EIF5A))

Andere Bezeichnung: EIF5A

Hintergrund: EIF5A, eukaryotic translation initiation factor 5A, EIF-5A, EIF5A1, MGC104255, MGC99547, eIF5AI, OTTHUMP00000128384, uORF A

Gen-ID: 1984

NCBI Accession: NP_001137232

Pathways: Regulation of Muscle Cell Differentiation