ACPP Antikörper (Internal Region)

Produktnummer ABIN570986

Der Ziege Polyklonal Anti-ACPP-Antikörper wurde für WB, IHC, ELISA und IF validiert. Er ist geeignet, ACPP in Proben von Human und Maus zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für ACPP Antikörper (Internal Region) (ABIN570986)

Target: ACPP (Acid Phosphatase, Prostate (ACPP))

Reaktivität: Human, Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser ACPP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Güteklasse: Verified

Produktdetails anti-ACPP Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: ACPP / PAP

Sequenz: YGIHKQKEKS RLQ

Spezifität: This antibody is expected to recognize both isoforms (NP_001090.2, NP_001127666.1).

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-YGIHKQKEKSRLQ

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: In paraffin embedded Human Prostate shows cytoplasm staining in some secretory cells of the gland. Recommended concentration, 3-5 μg/mL. This antibody has been successfully used in IHC on Mouse: Nousiainen HO et al. (2014) PMID: 2484

Western Blot: Approx 50 kDa band observed in Human Prostate lysates (calculated MW of 44.6 kDa according to NP_001090.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Patel et al, ,Clin Chem. 1986 O

Peptide ELISA: antibody detection limit dilution 1:64000.

Kommentare:

Immunofluorescence:: This antibody has been successfully used in IF on Mouse: Nousiainen HO et al. (2014) PMID: 24846136.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Referenzen für anti-ACPP Antikörper (ABIN570986)

Nousiainen, Quintero, Myöhänen, Voikar, Mijatovic, Segerstråle, Herrala, Kulesskaya, Pulkka, Kivinummi, Abo-Ramadan, Taira, Piepponen, Rauvala, Vihko: "Mice deficient in transmembrane prostatic acid phosphatase display increased GABAergic transmission and neurological alterations." in: PLoS ONE, Vol. 9, Issue 5, pp. e97851, (2014) (PubMed).

Details zu ACPP

Target: ACPP (Acid Phosphatase, Prostate (ACPP))

Andere Bezeichnung: ACP3

Hintergrund: ACPP, acid phosphatase, prostate, ACP-3, ACP3, PAP, prostatic acid phosphotase

Gen-ID: 55, 56318, 56780

NCBI Accession: NP_001090

Pathways: Synaptic Membrane, Ribonucleoside Biosynthetic Process