ITM2B Antikörper (Internal Region)

Produktnummer ABIN570955

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch ITM2B in ELISA, WB, IHC, FACS und IF. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für ITM2B Antikörper (Internal Region) (ABIN570955)

Target: ITM2B (Integral Membrane Protein 2B (ITM2B))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser ITM2B Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Güteklasse: Verified

Produktdetails anti-ITM2B Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: ITM2B

Sequenz: HDKETYKLQR RETIK

Kreuzreaktivität: Rind (Kuh), Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-HDKETYKLQRRETIK, from the internal region of the protein sequence according to NP_068839.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Human Small Intestine. Recommended concentration: 2.5 μg/mL.

Western Blot: Approx. 30-35 kDa band observed in HepG2 cell lysates (calculated MW of 30.3 kDa according to NP_068839.1). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Rat an

Peptide ELISA: antibody detection limit dilution 1:8000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and Golgi apparatus of A431 and HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recomme

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu ITM2B

Target: ITM2B (Integral Membrane Protein 2B (ITM2B))

Andere Bezeichnung: ITM2B

Hintergrund: ITM2B, integral membrane protein 2B, ABRI, BRI, BRI2, BRICD2B, E25B, E3-16, FBD, BRICHOS domain containing 2B, OTTHUMP00000018394

Gen-ID: 9445, 16432, 290364

NCBI Accession: NP_068839