OXT Antikörper (C-Term)

Produktnummer ABIN570836

Der Ziege Polyklonal Anti-OXT-Antikörper wurde für ELISA, IF und FACS validiert. Er ist geeignet, OXT in Proben von Maus zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für OXT Antikörper (C-Term) (ABIN570836)

Target: OXT (Oxytocin, Prepropeptide (OXT))

Reaktivität: Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser OXT Antikörper ist unkonjugiert

Applikation: ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-OXT Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: OXT (mouse)

Sequenz: DGCRTDPACD PES

Kreuzreaktivität: Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: DGCRTDPACDPES

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Preliminary experiments gave an approx 22 kDa band in Mouse Liver after 1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for this band, given the calculated size of 12.9 kDa according to NP

Peptide ELISA: antibody detection limit dilution 1:16000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and membranes of NIH3T3 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of NIH3T3 cells. Recommended concen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Referenzen für anti-OXT Antikörper (ABIN570836)

McMahon, Chang, Jackson: "Multiple cytosolic calcium buffers in posterior pituitary nerve terminals." in: The Journal of general physiology, Vol. 147, Issue 3, pp. 243-54, (2016) (PubMed).

Details zu OXT

Target: OXT (Oxytocin, Prepropeptide (OXT))

Andere Bezeichnung: Oxt

Hintergrund: Oxt, oxytocin, OT, Oxy, OTTMUSP00000016535

Gen-ID: 18429, 25504

NCBI Accession: NP_035155

Pathways: Myometrial Relaxation and Contraction, Feeding Behaviour