IKZF4 Antikörper (Internal Region)

Produktnummer ABIN570827

Der Ziege Polyklonal Anti-IKZF4-Antikörper wurde für WB, ELISA, IF und FACS validiert. Er ist geeignet, IKZF4 in Proben von Human und Maus zu detektieren.

Kurzübersicht für IKZF4 Antikörper (Internal Region) (ABIN570827)

Target: IKZF4 (IKAROS Family Zinc Finger 4 (Eos) (IKZF4))

Reaktivität: Human, Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser IKZF4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-IKZF4 Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: IKZF4

Sequenz: RPTFIDRLAN SLTKR

Spezifität: This antibody is expected to recognise reported isoforms NP_071910.3, NP_001338019.1, NP_001338020.1, NP_001338021.1

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-RPTFIDRLANSLTKR, from the internal region of the protein sequence according to NP_071910.3.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 60 kDa band observed in nuclear lysates of cell line Jurkat, approx.. 60+70 kDa in nuclear lysates of cell line K562, and approx. 55 kDa in Mouse Heart lysates (calculated MW of 64.1 kDa according to Human NP_071910.3, 59.1 kDa Human NP_0

Peptide ELISA: antibody detection limit dilution 1:2000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the nuclei of U2OS and HeLa cells. Recommended concentration: (5)μg/ml.

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 1

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu IKZF4

Target: IKZF4 (IKAROS Family Zinc Finger 4 (Eos) (IKZF4))

Andere Bezeichnung: IKZF4

Hintergrund: IKZF4, IKAROS family zinc finger 4 (Eos), EOS, KIAA1782, ZNFN1A4, zinc finger protein, subfamily 1A, 4, zinc finger protein, subfamily 1A, 4 (Eos), zinc finger transcription factor Eos

Gen-ID: 64375, 22781

NCBI Accession: NP_071910