TYR Antikörper

Produktnummer ABIN5708030

Dieses Kaninchen Monoklonal-Antikörper erkennt spezifisch TYR in IHC (p). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für TYR Antikörper (ABIN5708030)

Target: TYR (Tyrosinase (TYR))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser TYR Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: TYR-2024R

Produktdetails anti-TYR Antikörper

Aufreinigung: Purified

Reinheit: Protein A affinity chromatography

Immunogen: Recombinant protein was used as the immunogen for this recombinant Tyrosinase antibody.

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: The stated application concentrations are suggested starting points. Titration of the recombinant Tyrosinase antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the recombinant Tyrosinase antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Details zu TYR

Target: TYR (Tyrosinase (TYR))

Andere Bezeichnung: Tyrosinase

Hintergrund: Recognizes a cluster of proteins between 70-80  kDa, identified as tyrosinase. Occasionally a minor band at 55  kDa is also detected. This mAb shows no cross-reaction with MAGE-1 and tyrosinase-related protein 1, TRP-1/gp75. Tyrosinase is a copper-containing metalloglycoprotein that catalyzes several steps in the melanin pigment biosynthetic pathway, the hydroxylation of tyrosine to L-3,4-dihydroxy-phenylalanine (dopa), and the subsequent oxidation of dopa to dopaquinone. Mutations of the tyrosinase gene occur in various forms of albinism. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Staining of melanomas with this mAb shows tyrosinase in melanotic as well as amelanotic variants. This mAb is a useful marker for melanocytes and melanomas.