Keratin 10 Antikörper

Produktnummer ABIN5707750

Dieses Anti-Keratin 10-Antikörper ist ein Kaninchen Monoklonal-Antikörper zur Detektion von Keratin 10 in IHC (p). Geeignet für Human und Maus.

Kurzübersicht für Keratin 10 Antikörper (ABIN5707750)

Target: Keratin 10 (KRT10)

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser Keratin 10 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: KRT10-1990R

Produktdetails anti-Keratin 10 Antikörper

Aufreinigung: Purified

Reinheit: Protein A affinity chromatography

Immunogen: Skin extract from a Psoriasis patient was used as the immunogen for the recombinant Cytokeratin 10 antibody.

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the recombinant Cytokeratin 10 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunohistochemistry (FFPE): 0.1-0.2 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the recombinant Cytokeratin 10 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Details zu Keratin 10

Target: Keratin 10 (KRT10)

Andere Bezeichnung: KRT10 / Cytokeratin 10

Hintergrund: This mAb recognizes a protein of 56.5  kDa, identified as cytokeratin 10 (CK10). CK10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK10 strictly parallels the extent of differentiation, it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. However, CK10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger and more advanced tumors (greater than 2 cm, clinical stages II and III), CK10 is detected very frequently. Expression of CK10 is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts.