Histone H1 Antikörper

Produktnummer ABIN5707524

Der Kaninchen Monoklonal Anti-Histone H1-Antikörper wurde für IHC (p) validiert. Er ist geeignet, Histone H1 in Proben von Human zu detektieren.

Kurzübersicht für Histone H1 Antikörper (ABIN5707524)

Target: Histone H1

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser Histone H1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: OSHT-3R

Produktdetails anti-Histone H1 Antikörper

Aufreinigung: Purified

Reinheit: Protein A affinity chromatography

Immunogen: Recombinant full-length human protein was used as the immunogen for the recombinant Histone H1 antibody.

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: The stated application concentrations are suggested starting points. Titration of the recombinant Histone H1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the recombinant Histone H1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Details zu Histone H1

Target: Histone H1

Hintergrund: Five major families of histones exist: H1/H5, H2A, H2B, H3, and H4. Histones H2A, H2B, H3 and H4 are known as the core histones, while histones H1/H5 are known as the linker histones. The core histones all exist as dimers, which are similar in that they all possess the histone fold domain, three alpha helices linked by two loops. It is this helical structure that allows for interaction between distinct dimers, particularly in a head-tail fashion (also called the handshake motif). The linker histone H1 binds the nucleosome at the entry and exit sites of the DNA, thus locking the DNA into place and allowing the formation of higher order structure. [Wiki]