E2F8 Antikörper (Internal Region)
Kurzübersicht für E2F8 Antikörper (Internal Region) (ABIN570707)
Target
Alle E2F8 Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
Güteklasse
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Bindungsspezifität
- Internal Region
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Verwendungszweck
- E2F8
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Sequenz
- KSIESDRRKI NSAPS
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Kreuzreaktivität
- Rind (Kuh), Human, Maus
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Aufreinigung
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Immunogen
- Peptide with sequence C-KSIESDRRKINSAPS , from the internal region of the protein sequence according to NP_078956.2.
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Isotyp
- IgG
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Applikationshinweise
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Western Blot: Preliminary testing showed a band at approx 80 kDa in Human K562 and Mouse Neuro2a cell lysate after 0.3 μg/mL antibody staining. Primary incubation 1 hour at room temperature.
Peptide ELISA: antibody detection limit dilution 1:32000.
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Kommentare
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Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of A431 and U2OS cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended conc
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- 0.5 mg/mL
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Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handhabung
- Minimize freezing and thawing.
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Lagerung
- -20 °C
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Informationen zur Lagerung
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- E2F8 (E2F Transcription Factor 8 (E2F8))
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Andere Bezeichnung
- E2F8
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Hintergrund
- E2F8, E2F transcription factor 8, FLJ23311, E2F family member 8
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Gen-ID
- 79733, 108961
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NCBI Accession
- NP_078956
Target
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