Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

Histone H4 Antikörper (pSer1)

Dieses Anti-Histone H4-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Histone H4 in WB, IHC, IF, ChIP, DB, MA und FM. Geeignet für Human.
Rockland
Produktnummer ABIN5706885
Hersteller Produkt- Nr.: 600-401-i96

Kurzübersicht für Histone H4 Antikörper (pSer1) (ABIN5706885)

Target

Alle Histone H4 Antikörper anzeigen
Histone H4

Reaktivität

  • 330
  • 252
  • 248
  • 16
  • 14
  • 13
  • 13
  • 11
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
Human

Wirt

  • 370
  • 24
  • 2
Kaninchen

Klonalität

  • 341
  • 53
  • 2
Polyklonal

Konjugat

  • 217
  • 21
  • 14
  • 14
  • 14
  • 14
  • 14
  • 14
  • 7
  • 7
  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
  • 3
  • 2
Dieser Histone H4 Antikörper ist unkonjugiert

Applikation

  • 215
  • 104
  • 101
  • 98
  • 56
  • 52
  • 48
  • 47
  • 47
  • 38
  • 38
  • 23
  • 22
  • 14
  • 12
  • 8
  • 7
  • 6
  • 2
  • 2
  • 1
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Multiplex Assay (MA), Fluorescence Microscopy (FM)
  • Bindungsspezifität

    • 33
    • 30
    • 29
    • 28
    • 20
    • 19
    • 18
    • 16
    • 15
    • 14
    • 14
    • 12
    • 12
    • 12
    • 9
    • 9
    • 9
    • 9
    • 9
    • 9
    • 7
    • 6
    • 5
    • 5
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pSer1

    Hersteller Produkt- Nr.

    600-401-i96

    Hersteller

    Rockland

    Verwendungszweck

    Histone H4 phospho S1 Antibody

    Kreuzreaktivität (Details)

    This antibody reacts with human Histone H4.

    Produktmerkmale

    Synonyms: rabbit anti-Histone H4 pS1 antibody, H4pS1, HIST2H4B, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4K, HIST1H4L, HIST2H4, HIST2H4A, HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, histone cluster 4, H4, histone 4, H4

    Aufreinigung

    Anti-Histone H4 [p Ser1] was affinity purified from monospecific antiserum by immunoaffinity chromatography.

    Sterilität

    Sterile filtered

    Immunogen

    Immunogen: Histone H4 [p Ser1] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic phosphorylated peptide surrounding Serine 1 of human Histone H4.

    Immunogen Type: Conjugated Peptide

    Isotyp

    IgG
  • Applikationshinweise

    Immunohistochemistry Dilution: 1:50

    Application Note: Anti-Histone H4 [p Ser1] antibody is tested for Western Blot, Dot Blot, Chromatin Immunoprecipitation, Immunocytochemistry, and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~13 kDa corresponding to Histone H4 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.

    ChIP Dilution: 2-5 μg/million cells

    Western Blot Dilution: 1:1000

    IF Microscopy Dilution: 1:50

    Other: Dot Blot 0.1 μg/mL

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    0.89 mg/mL

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 30 % Glycerol

    Preservative: 0.01 % (w/v) Sodium Azide

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

    Haltbarkeit

    12 months
  • Target

    Histone H4

    Hintergrund

    Background: Chromatin is the arrangement of DNA and proteins in which chromosomes are formed. Correspondingly, chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is a very dynamic structure in which numerous post-translational modifications work together to activate or repress the availability of DNA to be copied, transcribed, or repaired. These marks decide which DNA will be open and commonly active (euchromatin) or tightly wound to prevent access and activation (heterochromatin). Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. In particular, phosphorylation of H4 Ser1 (H4 pS1) has been linked to mitosis and DNA repair. This modification is enriched in sites proximal to double stranded brakes, but not those associated with UV damage. Casein kinase II (CK2) phosphorylates H4 Ser1, and it also implicated in regulating the DNA damage response. Furthermore, recruitment of CK2 requires the SIN3/RPD3 histone deacetylase complex. Anti-Histone H4 are ideal for researchers interested in DNA Repair, Chromatin Modifiers, Chromatin Research, Histones and Modified Histones, DNA replication Transcription Translation and Splicing, and Epigenetics research.

    Gen-ID

    121504

    NCBI Accession

    NP_001029249

    UniProt

    P62805
Sie sind hier:
Chat with us!