Histone 3 Antikörper (pSer28)

Produktnummer ABIN5706879

Hersteller Produkt- Nr.: 600-401-i83

Der Kaninchen Polyklonal anti-Histone 3 Antikörper (ABIN5706879) detektiert spezifisch Histone 3 in WB, IHC, IF, ChIP, FM und MA. Dieser Antikörper reagiert spezifisch mit Proben aus Human und Maus.

Kurzübersicht für Histone 3 Antikörper (pSer28) (ABIN5706879)

Target: Histone 3 (H3) (Histone H3 (H3))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Histone 3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Fluorescence Microscopy (FM), Multiplex Assay (MA)

Produktdetails anti-Histone 3 Antikörper

Bindungsspezifität: pSer28

Hersteller Produkt- Nr.: 600-401-i83

Hersteller: Rockland

Verwendungszweck: Histone H3 phospho S28 Antibody

Kreuzreaktivität (Details): This antibody reacts with human Histone H3.

Produktmerkmale: Synonyms: rabbit anti-Histone H3 pS28 antibody, H3.3B, H3.3AH3F3H3F3B, H3 histone, family 3A, histone H3.3, MGC87782, MGC87783, H3pS28

Aufreinigung: Anti-Histone H3 [p Ser28] was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Histone H3 [p Ser28] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic phosphorylated peptide surrounding Serine 28 of human Histone H3.

Immunogen Type: Conjugated Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:150

Application Note: Anti-Histone H3 [p Ser28] antibody is tested for Western Blot and Immunofluorescence. This antibody is useful for Chromatin Immunoprecipitation, Dot Blot, and Immunocytochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.

ChIP Dilution: 2-5 μg/million cells

Western Blot Dilution: 1:500

IF Microscopy Dilution: 1:150

Other: Dot Blot 1:1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.96 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 30 % Glycerol

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu Histone 3

Target: Histone 3 (H3) (Histone H3 (H3))

Andere Bezeichnung: Histone H3

Hintergrund: Background: Chromatin is the arrangement of DNA and proteins in which chromosomes are formed. Correspondingly, chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is a very dynamic structure in which numerous post-translational modifications work together to activate or repress the availability of DNA to be copied, transcribed, or repaired. These marks decide which DNA will be open and commonly active (euchromatin) or tightly wound to prevent access and activation (heterochromatin). Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. In particular, phosphorylation of serine 28 of H3 (H3 pS28) is a mitotic and immediate early gene marker. Although similar to phosphorylation on serine 10 of H3 in the role of chromatin condensation in mitosis and the ability to respond to external stimuli such as stress and mitogen activation, these two phosphorylation sites are independent and promote gene expression separately. Phosphorylation of S28 is modulated by MSK1, MSK2, and Aurora-B kinase. Anti-Histone H3 are ideal for researchers interested in Chromatin Research, Epigenetics, Chromatin Modifiers, Histones and Modified Histones research.

Gen-ID: 126961

NCBI Accession: NP_001005464

UniProt: Q71DI3