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Histone H4 Antikörper (meLys20)

Der Rabbit Polyclonal Anti-Histone H4-Antikörper wurde für WB, IHC, IF, ChIP, DB, MA, FM validiert. Er ist geeignet, Histone H4 in Proben von Human, Mouse, C. elegans zu detektieren. Es ist 1 Publikation verfügbar.
Rockland
Produktnummer ABIN5706797
Hersteller Produkt- Nr.: 600-401-j01

Kurzübersicht für Histone H4 Antikörper (meLys20) (ABIN5706797)

Target

Alle Histone H4 Antikörper anzeigen
Histone H4

Reaktivität

  • 330
  • 252
  • 247
  • 16
  • 14
  • 13
  • 13
  • 11
  • 4
  • 4
  • 4
  • 4
  • 4
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
Human, Maus, C. elegans

Wirt

  • 370
  • 24
  • 2
Kaninchen

Klonalität

  • 341
  • 53
  • 2
Polyklonal

Konjugat

  • 217
  • 21
  • 14
  • 14
  • 14
  • 14
  • 14
  • 14
  • 7
  • 7
  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
  • 3
  • 2
Dieser Histone H4 Antikörper ist unkonjugiert

Applikation

  • 215
  • 104
  • 101
  • 98
  • 56
  • 52
  • 48
  • 47
  • 47
  • 38
  • 38
  • 23
  • 22
  • 14
  • 12
  • 8
  • 7
  • 6
  • 2
  • 2
  • 1
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Multiplex Assay (MA), Fluorescence Microscopy (FM)
  • Bindungsspezifität

    • 33
    • 30
    • 29
    • 28
    • 19
    • 19
    • 18
    • 16
    • 15
    • 14
    • 14
    • 13
    • 12
    • 12
    • 9
    • 9
    • 9
    • 9
    • 9
    • 9
    • 7
    • 6
    • 5
    • 5
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    meLys20

    Hersteller Produkt- Nr.

    600-401-j01

    Hersteller

    Rockland

    Verwendungszweck

    Histone H4 K20me1 Antibody

    Kreuzreaktivität (Details)

    This antibody reacts with human Histone H4.

    Produktmerkmale

    Synonyms: rabbit anti-Histone H4 monomethyl Lys20 antibody, H4K20me1, HIST2H4B, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4K, HIST1H4L, HIST2H4, HIST2H4A, HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, histone cluster 4, H4, histone 4, H4

    Aufreinigung

    Anti-Histone H4 [Monomethyl Lys20] was affinity purified from monospecific antiserum by immunoaffinity chromatography.

    Sterilität

    Sterile filtered

    Immunogen

    Immunogen: Histone H4 [Monomethyl Lys20] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic monomethylated peptide surrounding Lysine 20 of human Histone H4.

    Immunogen Type: Conjugated Peptide

    Isotyp

    IgG
  • Applikationshinweise

    Immunohistochemistry Dilution: 1:50

    Application Note: Anti-Histone H4 [Monomethyl Lys20] antibody is tested for Western Blot, Dot Blot, Chromatin Immunoprecipitation, Immunofluorescence, and Immunocytochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~13 kDa corresponding to Histone H4 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.

    ChIP Dilution: 2-5 μg/million cells

    Western Blot Dilution: 1:500

    IF Microscopy Dilution: 1:50

    Other: Dot Blot 1:1000

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    1.12 mg/mL

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 30 % Glycerol

    Preservative: 0.01 % (w/v) Sodium Azide

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

    Haltbarkeit

    12 months
  • Moiseeva, Hood, Schamus, OConnor, Conrads, Bakkenist: "ATR kinase inhibition induces unscheduled origin firing through a Cdc7-dependent association between GINS and And-1." in: Nature communications, Vol. 8, Issue 1, pp. 1392, (2018) (PubMed).

  • Target

    Histone H4

    Hintergrund

    Background: SET8 specifically catalyzes the mono-methylation of H4 at K20. Loss of this crucial methylation causes multiple DNA breaks, which instigates a p53-dependent DNA damage response to avoid mitosis and aberrant chromosomal activity. Therefore, this PTM is essential to genome replication and stability through S-phase. In mammalian stem cells, Xist expression blocks the formation of H4K20me1, which is one of the first examples of a direct connection between chromatin and stem cell differentiation. Anti-Histone H4 are ideal for researchers interested in Chromatin Modifiers, Chromatin Research, DNA Repair, DNA replication Transcription Translation and Splicing, Histones and Modified Histones, and Epigenetics research.

    Gen-ID

    121504

    NCBI Accession

    NP_001029249

    UniProt

    P62805
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