Histone H4 Antikörper (meLys20)
Kurzübersicht für Histone H4 Antikörper (meLys20) (ABIN5706797)
Target
Alle Histone H4 Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
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Bindungsspezifität
- meLys20
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Hersteller Produkt- Nr.
- 600-401-j01
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Hersteller
- Rockland
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Verwendungszweck
- Histone H4 K20me1 Antibody
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Kreuzreaktivität (Details)
- This antibody reacts with human Histone H4.
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Produktmerkmale
- Synonyms: rabbit anti-Histone H4 monomethyl Lys20 antibody, H4K20me1, HIST2H4B, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4K, HIST1H4L, HIST2H4, HIST2H4A, HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, histone cluster 4, H4, histone 4, H4
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Aufreinigung
- Anti-Histone H4 [Monomethyl Lys20] was affinity purified from monospecific antiserum by immunoaffinity chromatography.
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Sterilität
- Sterile filtered
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Immunogen
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Immunogen: Histone H4 [Monomethyl Lys20] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic monomethylated peptide surrounding Lysine 20 of human Histone H4.
Immunogen Type: Conjugated Peptide
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Isotyp
- IgG
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Applikationshinweise
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Immunohistochemistry Dilution: 1:50
Application Note: Anti-Histone H4 [Monomethyl Lys20] antibody is tested for Western Blot, Dot Blot, Chromatin Immunoprecipitation, Immunofluorescence, and Immunocytochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~13 kDa corresponding to Histone H4 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.
ChIP Dilution: 2-5 μg/million cells
Western Blot Dilution: 1:500
IF Microscopy Dilution: 1:50
Other: Dot Blot 1:1000
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- 1.12 mg/mL
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Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 30 % Glycerol
Preservative: 0.01 % (w/v) Sodium Azide -
Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Lagerung
- 4 °C,-20 °C
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Informationen zur Lagerung
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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Haltbarkeit
- 12 months
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ATR kinase inhibition induces unscheduled origin firing through a Cdc7-dependent association between GINS and And-1." in: Nature communications, Vol. 8, Issue 1, pp. 1392, (2018) (PubMed).
: "
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ATR kinase inhibition induces unscheduled origin firing through a Cdc7-dependent association between GINS and And-1." in: Nature communications, Vol. 8, Issue 1, pp. 1392, (2018) (PubMed).
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- Histone H4
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Hintergrund
- Background: SET8 specifically catalyzes the mono-methylation of H4 at K20. Loss of this crucial methylation causes multiple DNA breaks, which instigates a p53-dependent DNA damage response to avoid mitosis and aberrant chromosomal activity. Therefore, this PTM is essential to genome replication and stability through S-phase. In mammalian stem cells, Xist expression blocks the formation of H4K20me1, which is one of the first examples of a direct connection between chromatin and stem cell differentiation. Anti-Histone H4 are ideal for researchers interested in Chromatin Modifiers, Chromatin Research, DNA Repair, DNA replication Transcription Translation and Splicing, Histones and Modified Histones, and Epigenetics research.
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Gen-ID
- 121504
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NCBI Accession
- NP_001029249
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UniProt
- P62805
Target
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