Hemoglobin Subunit beta Antikörper (N-Term)

Produktnummer ABIN5706740

Hersteller Produkt- Nr.: 200-301-gs4

Der Maus Monoklonal Anti-Hemoglobin Subunit beta-Antikörper wurde für WB und ELISA validiert. Er ist geeignet, Hemoglobin Subunit beta in Proben von Human zu detektieren.

Kurzübersicht für Hemoglobin Subunit beta Antikörper (N-Term) (ABIN5706740)

Target: Hemoglobin Subunit beta (HBB) (Hemoglobin beta (HBB))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Hemoglobin Subunit beta Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Klon: 14G2-G11-F11

Produktdetails anti-Hemoglobin Subunit beta Antikörper

Bindungsspezifität: N-Term

Hersteller Produkt- Nr.: 200-301-gs4

Hersteller: Rockland

Verwendungszweck: Hemoglobin A (beta chain) Antibody

Kreuzreaktivität (Details): This protein A purified mouse monoclonal antibody reacts specifically with human HbA beta chain isoform.

Produktmerkmale: Synonyms: mouse anti-HbA antibody, mouse anti-hemoglobin antibody, Hemoglobin beta subunit, Hemoglobin subunit beta, Hemoglobin beta chain, HBB, HBA, Hbβ Antibody, LVV-hemorphin-7, Spinorphin, Sickle Cell Disease (SCD)

Aufreinigung: Anti-Hemoglobin beta β is purified from tissue culture supernatant by protein A purification.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Anti-Hemoglobin A (beta chain) Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues near the N-terminus of Hb beta-subunit conjugated to KLH.

Immunogen Type: Conjugated Peptide

Isotyp: IgG2a lambda

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-Hemoglobin A (beta chain) (MOUSE) antibody has been tested by ELISA and western blot. This antibody is designed for use in lateral flow. Specific conditions of reactivity should be optimized by the end user. Expect a band of approximately 16 kDa.

Western Blot Dilution: 1 μg/mL

ELISA Dilution: 1:20,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.00 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu Hemoglobin Subunit beta

Target: Hemoglobin Subunit beta (HBB) (Hemoglobin beta (HBB))

Andere Bezeichnung: HBB

Hintergrund: Background: HbA antibodies detect the hemoglobin beta subunit wild type variant A isoform. Functional adult hemoglobin (Hb) is a hetero tetramer composed of 2 alpha and 2 beta subunits (α2β2). Common isoform variants of hemoglobin include HbA, HbS, HbC, HbF, and HbA-2. Sickle cell disease (SCD), thalassemias and hemoglobinopathies occur when aberrant forms of hemoglobin are expressed in children and adults. Globin gene mutations affect the structure and expression levels of Hb. Sickle cell disease and the more benign sickle cell trait are observed in more than 100 million people globally. Perhaps the most significant mutation is the E6V in the beta subunit and the cause of SCD, but other relevant isoforms of Hb are observed. HbA antibody cross reacts with HbA-2 but does not react other forms Hb. This antibody is ideal for investigators involved in Cardiovascular and developmental biology research.

Gen-ID: 3043

NCBI Accession: NP_000509

UniProt: P68871