Ube2t Antikörper

Produktnummer ABIN5700967

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch Ube2t in WB, ELISA, IP und IF. Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte.

Kurzübersicht für Ube2t Antikörper (ABIN5700967)

Target: Ube2t (Ubiquitin-Conjugating Enzyme E2T (Ube2t))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Ube2t Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF)

Produktdetails anti-Ube2t Antikörper

Verwendungszweck: UBE2T antibody

Immunogen: ubiquitin-conjugating enzyme E2T (putative)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB : 1:200-1:2000,IP:1:200-1:2000,IF:1:50-1:500

Kommentare:

Jurkat cells were subjected to SDS PAGE followed by western blot with FNab09185(UBE2T antibody) at dilution of 1:800

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol  pH 7.3

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze / thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: -20°C for 12 months

Haltbarkeit: 12 months

Details zu Ube2t

Target: Ube2t (Ubiquitin-Conjugating Enzyme E2T (Ube2t))

Andere Bezeichnung: UBE2T

Hintergrund:

Synonyms: Ubiquitin-conjugating enzyme E2 T|Cell proliferation-inducing gene 50 protein|E2 ubiquitin-conjugating enzyme T|Ubiquitin carrier protein T|Ubiquitin-protein ligase T|UBE2T

Background: The ubiquitin(Ub)-mediated protein degradation pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub to specific protein substrates. The first step requires ATP-dependent activation of the C-terminus of Ub and the assembly of multi-Ubs by Ub-activating enzyme E1. The ubiquitin-conjugating enzyme E2, catalytic(UBCc) domain, is then conjugated to Ubs, through a thiol-ester linkage between a conserved cysteine and the C-terminus of Ub, to generate an intermediate Ub-E2 complex. Then the E3, a ligase, catalyzes the transfer of Ub from E2 to the appropriate substrate. This pathway regulates many fundamental cellular processes. There are also other E2s which form thiol-ester linkages without the use of E3s as well as several UBC homologs(TSG101, Mms2, Croc-1 and similar proteins), which lack the active site cysteine essential for ubiquitination and appear to function in DNA repair pathways.

Molekulargewicht: 24kDa

UniProt: Q9NPD8

Pathways: Chromatin Binding