Ube2t Antikörper

Produktnummer ABIN5700967

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Ube2t in WB, ELISA, IP und IF. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für Ube2t Antikörper (ABIN5700967)

Target: Ube2t (Ubiquitin-Conjugating Enzyme E2T (Ube2t))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Ube2t Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF)

Produktdetails anti-Ube2t Antikörper

Immunogen: ubiquitin-conjugating enzyme E2T (putative)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB : 1:200-1:2000,IP:1:200-1:2000,IF:1:50-1:500

Kommentare:

Jurkat cells were subjected to SDS PAGE followed by western blot with FNab09185(UBE2T antibody) at dilution of 1:800

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol  pH 7.3

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze / thaw cycles.

Lagerung: -20 °C

Haltbarkeit: 12 months

Details zu Ube2t

Target: Ube2t (Ubiquitin-Conjugating Enzyme E2T (Ube2t))

Andere Bezeichnung: UBE2T/HSPC150

Hintergrund: Synonyms:HSPC150, PIG50, UBE2T, Ubiquitin carrier protein T, Ubiquitin protein ligase T Background:The ubiquitin(Ub)-mediated protein degradation pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub to specific protein substrates. The first step requires ATP-dependent activation of the C-terminus of Ub and the assembly of multi-Ubs by Ub-activating enzyme E1. The ubiquitin-conjugating enzyme E2, catalytic(UBCc) domain, is then conjugated to Ubs, through a thiol-ester linkage between a conserved cysteine and the C-terminus of Ub, to generate an intermediate Ub-E2 complex. Then the E3, a ligase, catalyzes the transfer of Ub from E2 to the appropriate substrate. This pathway regulates many fundamental cellular processes. There are also other E2s which form thiol-ester linkages without the use of E3s as well as several UBC homologs(TSG101, Mms2, Croc-1 and similar proteins), which lack the active site cysteine essential for ubiquitination and appear to function in DNA repair pathways.

Molekulargewicht: 24kDa

UniProt: Q9NPD8

Pathways: Chromatin Binding