PKM1 Antikörper

Produktnummer ABIN5697233

Der Kaninchen-Polyklonal Anti-PKM1-Antikörper wurde für WB, IHC, ELISA, IP und IF validiert. Er ist geeignet zum Nachweis von PKM1 in Proben aus Human, Maus und Ratte. }

Kurzübersicht für PKM1 Antikörper (ABIN5697233)

Target: PKM1

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF)

Produktdetails

Verwendungszweck: PKM1-specific antibody

Immunogen: peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with FNab06494(PKM1-specific) at dilution of 1:1500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol  pH 7.3

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze / thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: -20°C for 12 months

Haltbarkeit: 12 months

Antigendetails

Target: PKM1

Hintergrund:

Synonyms: PKM1|PK-M1|PKM1 pyruvate kinase|muscle|PKM|Pyruvate kinase PKM

Background: PKM, also named as OIP3, PK2, PK3, PKM, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate(PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM plays a general role in caspase independent cell death of tumor cells. PKM has 2 isoforms named PKM1 and PKM2. By the upstream metabolite fructose-1,6-bisphosphate, PKM1 is a constitutively active enzyme.The primary pyruvate kinase isoform before tumour development is PK-M1, however, the primary isoform from four independent tumours is PK-M2(PMID:18337823). This antibody is specific to PKM1 isoform.