PI3 Antikörper (AA 61-117)

Produktnummer ABIN5693295

Dieses Maus Monoklonal-Antikörper erkennt spezifisch PI3 in IHC und ELISA. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für PI3 Antikörper (AA 61-117) (ABIN5693295)

Target: PI3 (Peptidase Inhibitor 3, Skin-Derived (PI3))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PI3 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), ELISA

Klon: C7

Produktdetails anti-PI3 Antikörper

Bindungsspezifität: AA 61-117

Verwendungszweck: Anti-Elafin/Skalp PI3 Antibody (monoclonal, C7)

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-Elafin/Skalp PI3 Antibody Picoband® (monoclonal, C7) (ABIN5693295). Tested in ELISA, IHC applications. This antibody reacts with Human.

Immunogen: E. coli-derived human Elafin/Skalp recombinant protein (Position: A61-Q117).

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
ELISA (Cap), 1-5 μg/mL1. "Entrez Gene: PI3 peptidase inhibitor 3, skin-derived (SKALP)". 2. Clauss A, Lilja H, Lundwall A (Nov 2002). "A locus on human chromosome 20 contains several genes expressing protease inhibitor domains with homology to whey acidic protein". Biochem J 368 (Pt 1): 233-42. 3. Molhuizen HO, Zeeuwen PL, Olde Weghuis D, Geurts van Kessel A, Schalkwijk J (Feb 1994). "Assignment of the human gene encoding the epidermal serine proteinase inhibitor SKALP (PI3) to chromosome region 20q12→q13". Cytogenet Cell Genet 66 (2): 129-31.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu PI3

Target: PI3 (Peptidase Inhibitor 3, Skin-Derived (PI3))

Andere Bezeichnung: PI3

Hintergrund:

Synonyms: Elafin, Elastase-specific inhibitor, ESI, Peptidase inhibitor 3, PI-3, Protease inhibitor WAP3, Skin-derived antileukoproteinase, SKALP, WAP four-disulfide core domain protein 14, PI3, WAP3, WFDC14

Tissue Specificity: Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.

Background: Elafin, also known as peptidase inhibitor 3 or skin-derived antileukoprotease (SKALP), is a protein that in humans is encoded by the PI3 gene. This gene encodes an elastase-specific inhibitor that functions as an antimicrobial peptide against Gram-positive and Gram-negative bacteria, and fungal pathogens. The protein contains a WAP-type four-disulfide core (WFDC) domain, and is thus a member of the WFDC domain family. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. And this gene belongs to the centromeric cluster. Expression of this gene is upgulated by bacterial lipopolysaccharides and cytokines.

Molekulargewicht: 25 kDa

Gen-ID: 5266

UniProt: P19957