NEDD4-2 Antikörper (AA 703-975)

Produktnummer ABIN5693274

Produktdetails anti-NEDD4-2 Antikörper

Target: NEDD4-2 (NEDD4L) (E3 ubiquitin-protein ligase NEDD4-like (NEDD4L))

Bindungsspezifität: AA 703-975

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NEDD4-2 Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB)

Marke: Picoband™

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for NEDD4-2 detection. Tested with WB, Direct ELISA in Human,Mouse,Rat.

Immunogen: E. coli-derived human NEDD4-2 recombinant protein (Position: N703-D975).

Anwendungsinformationen

Applikationshinweise:

Recommended Detection Systems: Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Application Details: Western blot, 0.1-0.5 μg/mL
Direct ELISA, 0.1-0.5 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Details zu NEDD4-2

Target: NEDD4-2 (NEDD4L) (E3 ubiquitin-protein ligase NEDD4-like (NEDD4L))

Andere Bezeichnung: NEDD4L (NEDD4L Produkte)

Synonyme: NEDD4-2 antikoerper, NEDD4.2 antikoerper, RSP5 antikoerper, hNEDD4-2 antikoerper, nedd4 antikoerper, nedd4-2 antikoerper, NEDD4 antikoerper, Nedd4-2 antikoerper, 1300012C07Rik antikoerper, Nedd4b antikoerper, neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase antikoerper, NEDD4L antikoerper, neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase S homeolog antikoerper, neural precursor cell expressed, developmentally down-regulated gene 4-like antikoerper, NEDD4L antikoerper, LOC776799 antikoerper, nedd4l.S antikoerper, Nedd4l antikoerper

Hintergrund:

Synonyms: E3 ubiquitin-protein ligase NEDD4-like, HECT-type E3 ubiquitin transferase NED4L, NEDD4.2, Nedd4-2, NEDD4L, KIAA0439, NEDL3

Tissue Specificity: Ubiquitously expressed, with highest levels in prostate, pancreas and kidney (PubMed:14615060, PubMed:15496141, PubMed:19664597). Expressed in melanocytes (PubMed:23999003).

Background: Neural precursor cell expressed developmentally downregulated gene 4-like (NEDD4L) or NEDD4-2 (NEDD4-2) is an enzyme (ubiquitin ligase) of the NEDD4 family. This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein mediates the ubiquitination of multiple target substrates and plays a critical role in epithelial sodium transport by regulating the cell surface expression of the epithelial sodium channel, ENaC. Single nucleotide polymorphisms in this gene may be associated with essential hypertension. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Pathways: Negative Regulation of Transporter Activity