MYLK Antikörper (AA 1441-1709)
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- Target Alle MYLK Antikörper anzeigen
- MYLK (Myosin Light Chain Kinase (MYLK))
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Bindungsspezifität
- AA 1441-1709
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Reaktivität
- Human, Maus, Ratte
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser MYLK Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- Verwendungszweck
- Anti-Myosin light chain kinase/MYLK Antibody Picoband®
- Kreuzreaktivität (Details)
- No cross-reactivity with other proteins.
- Produktmerkmale
- Anti-Myosin light chain kinase/MYLK Antibody Picoband® (ABIN5693029). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- Immunogen
- E. coli-derived human MYLK recombinant protein (Position: D1441-D1709).
- Isotyp
- IgG
- Top Product
- Discover our top product MYLK Primärantikörper
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- Applikationshinweise
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Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
ELISA, 0.1-0.5 μg/mL
1. "Entrez Gene: MYLK myosin, light chain kinase". 2. Connell LE, Helfman DM (2007). "Myosin light chain kinase plays a role in the regulation of epithelial cell survival". J. Cell. Sci. 119 (Pt 11): 2269-81. - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- Konzentration
- 500 μg/mL
- Buffer
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- Target
- MYLK (Myosin Light Chain Kinase (MYLK))
- Andere Bezeichnung
- MYLK (MYLK Produkte)
- Hintergrund
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Synonyms: Myosin light chain kinase, smooth muscle, MLCK, s mMLCK, Kinase-related protein, KRP, Telokin, Myosin light chain kinase, smooth muscle, deglutamylated form, MYLK, MLCK, MLCK1, MYLK1
Tissue Specificity: Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. Telokin has been found in a wide variety of adult and fetal tissues. Accumulates in well differentiated enterocytes of the intestinal epithelium in response to tumor necrosis factor (TNF).
Background: Myosin light chain kinase, smooth muscle also known as kinase-related protein (KRP) or telokin is an enzyme that in humans is encoded by the MYLK gene. This gene, a muscle member of the immunoglobulin gene superfamily, encodes myosin light chain kinase which is a calcium/calmodulin dependent enzyme. This kinase phosphorylates myosin regulatory light chains to facilitate myosin interaction with actin filaments to produce contractile activity. This gene encodes both smooth muscle and nonmuscle isoforms. In addition, using a separate promoter in an intron in the 3' region, it encodes telokin, a small protein identical in sequence to the C-terminus of myosin light chain kinase, that is independently expressed in smooth muscle and functions to stabilize unphosphorylated myosin filaments. A pseudogene is located on the p arm of chromosome 3. Four transcript variants that produce four isoforms of the calcium/calmodulin dependent enzyme have been identified as well as two transcripts that produce two isoforms of telokin.
- Molekulargewicht
- 250 kDa
- Gen-ID
- 4638
- UniProt
- Q15746
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