Cytokeratin 19 Antikörper (C-Term)

Produktnummer ABIN5693023

Der Maus Monoklonal Anti-Cytokeratin 19-Antikörper wurde für WB, IHC und IF validiert. Er ist geeignet, Cytokeratin 19 in Proben von Human zu detektieren. Es sind 5+ Publikationen verfügbar.

Kurzübersicht für Cytokeratin 19 Antikörper (C-Term) (ABIN5693023)

Target: Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cytokeratin 19 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Klon: 3D4

Produktdetails anti-Cytokeratin 19 Antikörper

Bindungsspezifität: AA 334-372, C-Term

Verwendungszweck: Anti-Cytokeratin 19 KRT19 Antibody Picoband® (monoclonal, 3D4)

Sequenz: QLAHIQALIS GIEAQLGDVR ADSERQNQEY QRLMDIKSR

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-Cytokeratin 19 KRT19 Antibody Picoband® (monoclonal, 3D4) (ABIN5693023). Tested in IF, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Cytokeratin 19, different from the related mouse and rat sequences by nine amino acids.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunofluorescence, 2 μg/mL1. "Entrez Gene: KRT19 keratin 19". 2. Schweizer J, Bowden PE, Coulombe PA, Langbein L, Lane EB, Magin TM, Maltais L, Omary MB, Parry DA, Rogers MA, Wright MW (Jul 2006). "New consensus nomenclature for mammalian keratins". J Cell Biol 174 (2): 169-74. 3. W. Jeffrey Allard, Jeri Matera, M. Craig Miller, et al. (October 2004). "Tumor Cells Circulate in the Peripheral Blood of All Major Carcinomas but not in Healthy Subjects or Patients With Nonmalignant Diseases". Clin. Cancer Research 10 (20): 6897-6904.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Referenzen für anti-Cytokeratin 19 Antikörper (ABIN5693023)

Wang, Said, Wang, Qu, Xu, Liu, Shen: "Establishment and evaluation of the goose embryo epithelial (GEE) cell line as a new model for propagation of avian viruses." in: PLoS ONE, Vol. 13, Issue 3, pp. e0193876, (2018) (PubMed).

Quan, Du, Hou, Wang, Zhang: "Utilization of E-cadherin by monocytes from tumour cells plays key roles in the progression of bone invasion by oral squamous cell carcinoma." in: Oncology reports, Vol. 38, Issue 2, pp. 850-858, (2017) (PubMed).

Jeng, Jeng, Jeng, Sheen, Li, Lu, Chang: "Tropism of liver epithelial cells toward hepatocellular carcinoma in vitro and in vivo with altering gene expression of cancer stem cells." in: American journal of surgery, Vol. 215, Issue 4, pp. 735-743, (2017) (PubMed).

Li, Zhang, Zhang, Liu, Qi, Zhao, Jiang, Zhai, Ji, Luo: "Stem cell-like circulating tumor cells indicate poor prognosis in gastric cancer." in: BioMed research international, Vol. 2014, pp. 981261, (2015) (PubMed).

Lu, Gu, Xu, Liu, Xie, Song: "Adult islets cultured in collagen gel transdifferentiate into duct-like cells." in: World journal of gastroenterology, Vol. 11, Issue 22, pp. 3426-30, (2005) (PubMed).

Details zu Cytokeratin 19

Target: Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

Andere Bezeichnung: KRT19

Hintergrund:

Synonyms: Keratin, type I cytoskeletal 19, Cytokeratin-19, CK-19, Keratin-19, K19, KRT19

Tissue Specificity: Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin.

Background: Keratin, type I cytoskeletal 19 is a protein that in humans is encoded by the KRT19 gene. The protein encoded by this gene is a member of the keratin family. It is specifically expressed in the periderm, the transiently superficial layer that envelops the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. Due to its high sensitivity, KRT19 is the most used marker for the RT-PCR-mediated detection of tumor cells disseminated in lymph nodes, peripheral blood, and bone marrow of breast cancer patients. Keratin 19 is often used together with keratin 8 and keratin 18 to differentiate cells of epithelial origin from hematopoietic cells in tests that enumerate circulating tumor cells in blood.

Molekulargewicht: 44 kDa

Gen-ID: 3880

UniProt: P08727