ADAM10 Antikörper (AA 214-325)

Produktnummer ABIN5692975

Produktdetails anti-ADAM10 Antikörper

Target: ADAM10 (ADAM Metallopeptidase Domain 10 (ADAM10))

Bindungsspezifität: AA 214-325

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ADAM10 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Verwendungszweck: Anti-ADAM10 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-ADAM10 Antibody Picoband® (ABIN5692975). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Immunogen: E. coli-derived human ADAM10 recombinant protein (Position: T214-D325).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL
ELISA, 0.1-0.5 μg/mL
1. Lunn, C. A., Fan, X., Dalie, B., Miller, K., Zavodny, P. J., Narula, S. K., Lundell, D.Purification of ADAM 10 from bovine spleen as a TNFalpha convertase.FEBS Lett. 400: 333-335, 1997. 2. Wolfsberg, T. G., Primakoff, P., Myles, D. G., White, J. M.ADAM, a novel family of membrane proteins containing a disintegrin and metalloprotease domain: multipotential functions in cell-cell and cell-matrix interactions.J. Cell Biol. 131: 275-278, 1995. 3. Yamazaki, K., Mizui, Y., Tanaka, I.Radiation hybrid mapping of human ADAM10 gene to chromosome 15.Genomics 45: 457-459, 1997.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Referenzen für anti-ADAM10 Antikörper (ABIN5692975)

Lin, Huang, Xie, Zhou, Yang, Huang: "Gemcitabine inhibits immune escape of pancreatic cancer by down regulating the soluble ULBP2 protein." in: Oncotarget, Vol. 7, Issue 43, pp. 70092-70099, (2018) (PubMed).

Details zu ADAM10

Target: ADAM10 (ADAM Metallopeptidase Domain 10 (ADAM10))

Andere Bezeichnung: ADAM10 (ADAM10 Produkte)

Hintergrund:

Synonyms: Disintegrin and metalloproteinase domain-containing protein 10, ADAM 10, CDw156, Kuzbanian protein homolog, Mammalian disintegrin-metalloprotease, CD156c, ADAM10, KUZ, MADM

Tissue Specificity: Expressed in spleen, lymph node, thymus, peripheral blood leukocyte, bone marrow, cartilage, chondrocytes and fetal liver.

Background: ADAM10, A Disintegrin and Metalloproteinase Domain 10, is also known as AD10. ADAM10 is a member of the ADAM family and members of this family are cell surface proteins with a unique structure possessing both potential adhesion and protease function. The ADAM10 gene is mapped to chromosome 15q21.3-q23. ADAM proteins contain an N-terminal signal sequence, followed by a prodomain, a metalloprotease-like domain, a disintegrin-like domain, a cysteine-rich region, an EGF -like repeat, a transmembrane domain, and a C-terminal cytoplasmic tail. Conversion of the membrane-bound precursor to a secreted mature protein is mediated by a protease termed TNFA convertase. ADAM10 possesses TNFA convertase activity.

Molekulargewicht: 72 kDa

Gen-ID: 102

UniProt: O14672

Pathways: Notch Signalweg, EGFR Signaling Pathway