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Splicing Factor 1 Antikörper (AA 160-266)

Dieses Anti-Splicing Factor 1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Splicing Factor 1 in WB, IHC, ELISA, IF, ICC und FACS. Geeignet für Human, Maus und Ratte.
Produktnummer ABIN5692946

Kurzübersicht für Splicing Factor 1 Antikörper (AA 160-266) (ABIN5692946)

Target

Alle Splicing Factor 1 (SF1) Antikörper anzeigen
Splicing Factor 1 (SF1)

Reaktivität

  • 59
  • 50
  • 18
  • 12
  • 10
  • 10
  • 8
  • 7
  • 7
  • 6
  • 5
  • 5
  • 3
  • 2
  • 1
  • 1
Human, Maus, Ratte

Wirt

  • 69
  • 5
Kaninchen

Klonalität

  • 70
  • 4
Polyklonal

Konjugat

  • 48
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser Splicing Factor 1 Antikörper ist unkonjugiert

Applikation

  • 64
  • 29
  • 24
  • 16
  • 13
  • 13
  • 13
  • 10
  • 8
  • 6
  • 3
  • 2
  • 2
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)
  • Bindungsspezifität

    • 29
    • 10
    • 7
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 160-266

    Verwendungszweck

    Anti-splicing factor 1/SF1 Antibody Picoband®

    Kreuzreaktivität (Details)

    No cross-reactivity with other proteins.

    Produktmerkmale

    Anti-splicing factor 1/SF1 Antibody Picoband® (ABIN5692946). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    Immunogen

    E. coli-derived human splicing factor 1 recombinant protein (Position: R160-Q266).

    Isotyp

    IgG
  • Applikationshinweise

    Western blot, 0.1-0.5 μg/mL
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
    Immunocytochemistry/Immunofluorescence, 2 μg/mL
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells
    ELISA, 0.1-0.5 μg/mL
    1. Toda, T., Iida, A., Miwa, T., Nakamura, Y., Imai, T. Isolation and characterization of a novel gene encoding nuclear protein at a locus (D11S636) tightly linked to multiple endocrine neoplasia type 1 (MEN1). Hum. Molec. Genet. 3: 465-470, 1994. 2. Caslini, C., Spinelli, O., Cazzaniga, G., Golay, J., De Gioia, L., Pedretti, A., Breviario, F., Amaru, R., Barbui, T., Biondi, A., Introna, M., Rambaldi, A. Identification of two novel isoforms of the ZNF162 gene: a growing family of signal transduction and activator of RNA proteins. Genomics 42: 268-277, 1997.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    Konzentration

    500 μg/mL

    Buffer

    Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • Target

    Splicing Factor 1 (SF1)

    Andere Bezeichnung

    SF1

    Hintergrund

    Synonyms: Splicing factor 1, Mammalian branch point-binding protein, BBP, mBBP, Transcription factor ZFM1, Zinc finger gene in MEN1 locus, Zinc finger protein 162, SF1, ZFM1, ZNF162

    Tissue Specificity: Detected in lung, ovary, adrenal gland, colon, kidney, muscle, pancreas, thyroid, placenta, brain, liver and heart.

    Background: Splicing factor 1 also known as zinc finger protein 162 (ZFM162) is a protein that in humans is encoded by the SF1 gene. This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The encoded protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants.

    Molekulargewicht

    68-80 kDa

    Gen-ID

    7536

    UniProt

    Q15637

    Pathways

    Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Maintenance of Protein Location
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