Splicing Factor 1 Antikörper (AA 160-266)
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- Target Alle Splicing Factor 1 (SF1) Antikörper anzeigen
- Splicing Factor 1 (SF1)
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Bindungsspezifität
- AA 160-266
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Reaktivität
- Human, Maus, Ratte
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser Splicing Factor 1 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)
- Verwendungszweck
- Anti-splicing factor 1/SF1 Antibody Picoband®
- Kreuzreaktivität (Details)
- No cross-reactivity with other proteins.
- Produktmerkmale
- Anti-splicing factor 1/SF1 Antibody Picoband® (ABIN5692946). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- Immunogen
- E. coli-derived human splicing factor 1 recombinant protein (Position: R160-Q266).
- Isotyp
- IgG
- Top Product
- Discover our top product SF1 Primärantikörper
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- Applikationshinweise
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Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x106 cells
ELISA, 0.1-0.5 μg/mL
1. Toda, T., Iida, A., Miwa, T., Nakamura, Y., Imai, T. Isolation and characterization of a novel gene encoding nuclear protein at a locus (D11S636) tightly linked to multiple endocrine neoplasia type 1 (MEN1). Hum. Molec. Genet. 3: 465-470, 1994. 2. Caslini, C., Spinelli, O., Cazzaniga, G., Golay, J., De Gioia, L., Pedretti, A., Breviario, F., Amaru, R., Barbui, T., Biondi, A., Introna, M., Rambaldi, A. Identification of two novel isoforms of the ZNF162 gene: a growing family of signal transduction and activator of RNA proteins. Genomics 42: 268-277, 1997. - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- Konzentration
- 500 μg/mL
- Buffer
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- Target
- Splicing Factor 1 (SF1)
- Andere Bezeichnung
- SF1 (SF1 Produkte)
- Hintergrund
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Synonyms: Splicing factor 1, Mammalian branch point-binding protein, BBP, mBBP, Transcription factor ZFM1, Zinc finger gene in MEN1 locus, Zinc finger protein 162, SF1, ZFM1, ZNF162
Tissue Specificity: Detected in lung, ovary, adrenal gland, colon, kidney, muscle, pancreas, thyroid, placenta, brain, liver and heart.
Background: Splicing factor 1 also known as zinc finger protein 162 (ZFM162) is a protein that in humans is encoded by the SF1 gene. This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The encoded protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants.
- Molekulargewicht
- 68-80 kDa
- Gen-ID
- 7536
- UniProt
- Q15637
- Pathways
- Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Maintenance of Protein Location
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