MYF5 Antikörper (AA 25-146)

Produktnummer ABIN5692895

Dieses Anti-MYF5-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von MYF5 in WB und ELISA. Geeignet für Human, Maus und Ratte.

Kurzübersicht für MYF5 Antikörper (AA 25-146) (ABIN5692895)

Target: MYF5 (Myogenic Factor 5 (MYF5))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MYF5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-MYF5 Antikörper

Bindungsspezifität: AA 25-146

Verwendungszweck: Anti-Myf5 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-Myf5 Antibody Picoband® (ABIN5692895). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Immunogen: E. coli-derived human Myf5 recombinant protein (Position: E25-Y146).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL
ELISA, 0.1-0.5 μg/mL
1. Gustafsson MK, Pan H, Pinney DF, Liu Y, Lewandowski A, Epstein DJ, Emerson CP (January 2002). "Myf5 is a target of long-range Shh signaling and Gli regulation for muscle specification". Genes & Development. 16 (1): 114-26. 2. Ott MO, Bober E, Lyons G, Arnold H, Buckingham M (April 1991). "Early expression of the myogenic regulatory gene, myf-5, in precursor cells of skeletal muscle in the mouse embryo". Development. 111 (4): 1097-107.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu MYF5

Target: MYF5 (Myogenic Factor 5 (MYF5))

Andere Bezeichnung: MYF5

Hintergrund:

Synonyms: Myogenic factor 5, Myf-5, Class C basic helix-loop-helix protein 2, bHLHc2, MYF5, BHLHC2

Tissue Specificity: Ubiquitously expressed. Present at highest levels in the brain, at high levels in the placenta and testis, at intermediate levels in the intestine, ovary, skeletal muscle and thymus and at lower levels in heart, kidney, liver, lung, pancreas, prostate and spleen. In the kidney, it is widely expressed in tubules, but sparsely expressed in the glomerulus (PubMed:24676636). Expression is significantly increased in renal biopsy specimens from idiopathic FSGS (PubMed:24676636). Overexpressed in many tumor types including breast, colorectal, endometrial, hepatic, kidney, lung, ovarian and pancreatic tumors.

Background: Myogenic factor 5 is a protein that in humans is encoded by the MYF5 gene. It is a protein with a key role in regulating muscle differentiation or myogenesis, specifically the development of skeletal muscle. Myf5 belongs to a family of proteins known as myogenic regulatory factors (MRFs). This transcription factor is the earliest of all MRFs to be expressed in the embryo, where it is only markedly expressed for a few days (specifically around 8 days post-somite formation and lasting until day 14 post-somite in mice). It functions during that time to commit myogenic precursor cells to become skeletal muscle. In fact, its expression in proliferating myoblasts has led to its classification as a determination factor. Furthermore, Myf5 is a master regulator of muscle development, possessing the ability to induce a muscle phenotype upon its forced expression in fibroblastic cells.

Molekulargewicht: 28 kDa

Gen-ID: 4617

UniProt: P13349

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development