Estrogen Receptor alpha Antikörper (Domain B)

Produktnummer ABIN5691094

Der Maus Monoklonal Anti-Estrogen Receptor alpha-Antikörper wurde für WB, IP und ICC validiert. Er ist geeignet, Estrogen Receptor alpha in Proben von Human zu detektieren.

Kurzübersicht für Estrogen Receptor alpha Antikörper (Domain B) (ABIN5691094)

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Estrogen Receptor alpha Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Klon: AER304

Produktdetails anti-Estrogen Receptor alpha Antikörper

Bindungsspezifität: AA 120-170, Domain B

Spezifität: The antibody AER304 is directed against estrogen receptor alpha (ERα) and recognises amino acids residues 120 - 170, an epitope located in the B domain of ERα.

Immunogen: AER304 is a mouse monoclonal IgG1 antibody derived by fusion of Sp2/0-Ag14 mouse myeloma cells with splenocytes from a BALB/c mouse, immunized with estrogen receptor protein purified from calf uterus.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: The AER304 antibody is suitable for the detection of estrogen receptor alpha by western blotting, immunoprecipitation and immunocytochemistry. In western blotting, the antibody recognises a doublet of approximately 67 kDa in non-reducing conditions. Optimal antibody dilutions for the different applications should be determined by titration.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Each vial contains 500 μL 0.2 mg/mL purified monoclonal antibody in phosphated buffered saline (PBS) containing 50 % glycerol + 0.2 % bovine serum albumin (BSA) + 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C, or in small aliquots at -20°C.

Details zu Estrogen Receptor alpha

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Andere Bezeichnung: Estrogen Receptor alpha/ERalpha/ERS1

Hintergrund: Estrogen receptor (ER) is a hormone binding molecule, which belongs to the nuclear hormone family of intracellular receptors. Estrogen receptors exist as alpha and beta forms encoded by separate genes ESR1 and ESR2. ERα and ERβ share a high degree of sequence homology and are activated by the hormone 17β-estradiol and related compounds, including phytoestrogens and xenoestrogens. Both ERα and ERβ function as transcription factors which mediate many of the biological effects of estradiol at the level of gene regulation. Both receptors and are involved in the normal development and function of numerous tissues. Studies also suggest that ER's may be involved in a range of pathological conditions including cancers, neurodegenerative diseases and cardiovascular disease.

UniProt: P03372

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly