Follistatin Antikörper (Biotin)

Produktnummer ABIN5690529

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Follistatin in WB und ELISA. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Follistatin Antikörper (Biotin) (ABIN5690529)

Target: Follistatin (FST)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Follistatin Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-Follistatin Antikörper (Biotin)

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hFollistatin. Human Follistatin specific antibody was purified by affinity chromatography and then biotinylated.

Anwendungsinformationen

Applikationshinweise: ELISA:
Direct:
To detect hFollistatin by direct ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFollistatin.

Sandwich
To detect hFollistatin by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human Follistatin as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFollistatin.

Western Blot:
To detect hFollistatin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFollistatin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Lagerung: -20 °C

Informationen zur Lagerung: Follistatin antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Details zu Follistatin

Target: Follistatin (FST)

Andere Bezeichnung: Follistatin

Hintergrund: Follistatin is a single-chain gonadal protein that specifically inhibits follicle-stimulating hormone release. The single FST gene encodes two isoforms, FST317 and FST344 containing 317 and 344 amino acids respectively, resulting from alternative splicing of the precursor mRNA. There is evidence linking polycystic ovary syndrome and follistatin. Follistatin binds directly to activin and functions as an activin antagonist. It specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone

Gen-ID: 10468

UniProt: P19883

Pathways: Negative Regulation of Hormone Secretion