Follistatin Antikörper

Produktnummer ABIN5690528

Dieses Anti-Follistatin-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Follistatin in WB, ELISA und Neut. Geeignet für Human.

Kurzübersicht für Follistatin Antikörper (ABIN5690528)

Target: Follistatin (FST)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Follistatin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Neutralization (Neut)

Produktdetails anti-Follistatin Antikörper

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hFollistatin. Human Follistatin specific antibody was purified by affinity chromatography employing immobilized hFollistatin matrix.

Anwendungsinformationen

Applikationshinweise: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Human Follistatin (100.0 ng/mL), a concentration of 8.0-12.0 μ,g/mL of this antibody is required.

ELISA:
Indirect:
To detect hFollistatin by indirect ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFollistatin.

Sandwich
To detect hFollistatin by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human Follistatin as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFollistatin.

Western Blot:
To detect hFollistatin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFollistatin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Lagerung: -20 °C

Informationen zur Lagerung: Follistatin antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Details zu Follistatin

Target: Follistatin (FST)

Andere Bezeichnung: Follistatin

Hintergrund: Follistatin is a single-chain gonadal protein that specifically inhibits follicle-stimulating hormone release. The single FST gene encodes two isoforms, FST317 and FST344 containing 317 and 344 amino acids respectively, resulting from alternative splicing of the precursor mRNA. There is evidence linking polycystic ovary syndrome and follistatin. Follistatin binds directly to activin and functions as an activin antagonist. It specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone

Gen-ID: 10468

UniProt: P19883

Pathways: Negative Regulation of Hormone Secretion