CCL4 Antikörper

Produktnummer ABIN5690510

Dieses Anti-CCL4-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von CCL4 in WB, ELISA und Neut. Geeignet für Maus.

Kurzübersicht für CCL4 Antikörper (ABIN5690510)

Target: CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CCL4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Neutralization (Neut)

Produktdetails anti-CCL4 Antikörper

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mMIP-1β,. Murine MIP-1β, specific antibody was purified by affinity chromatography employing immobilized mMIP-1β, matrix.

Anwendungsinformationen

Applikationshinweise: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Murine MIP-1β, (100.0 ng/mL), a concentration of 8.0-10.0 μ,g/mL of this antibody is required.

ELISA:
Indirect:
To detect mMIP-1b by indirect ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMIP-1b.

Sandwich
To detect mMIP-1b by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Murine MIP-1b as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMIP-1b.

Western Blot:
To detect mMIP-1b by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMIP-1b is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Lagerung: -20 °C

Informationen zur Lagerung: MIP-1B antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Details zu CCL4

Target: CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))

Andere Bezeichnung: MIP-1B

Gen-ID: 20303

UniProt: P14097