ELISA: (direct and indirect: 1:2,000-1:5,000). Western Blot: (1:2,000-1:5,000 using ECL, suggested blocking and dilution buffer is PBST containing 0.05 % Tween 20 and 5 % skim milk, suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
1 mg/mL
Buffer
Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.
Konservierungsmittel
Without preservative
Lagerung
-20 °C
Informationen zur Lagerung
Stable for at least 6 months after receipt when stored at -20°C.
Target
IL1RL1
(Interleukin 1 Receptor-Like 1 (IL1RL1))
Andere Bezeichnung
ST2
Hintergrund
The ST2 gene was originally identified as a gene induced by serum or oncogene expression in fibroblasts. The gene produces a shorter soluble secreted form (ST2) and a longer, transmembrane form (ST2L) by alternative splicing. Soluble ST2 has been shown to downregulate the expression of TLR1 and TLR4. ST2L negatively regulates TLR4 signaling and induces endotoxin tolerance, and enhances Th2 responses. IL-33 is the specific ligand for ST2L.