Der Biotin-konjugierte Kaninchen Polyklonal anti-CCL27 Antikörper (ABIN5690483) detektiert spezifisch CCL27 in WB und ELISA.
Dieser Antikörper reagiert spezifisch mit Proben aus Human.
Produced from sera of rabbits immunized with highly pure recombinant Human CTACK. Human CTACK specific antibody was purified by affinity chromatography and then biotinylated.
ELISA: Direct: To detect Human CTACK by direct ELISA (using 100 μ,L/well) a concentration of approximately 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human CTACK.
Sandwich To detect Human CTACK by sandwich ELISA (using 100 μ,L/well) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci's Polyclonal Anti-Human CTACK (38-263) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human CTACK.
Western Blot:
To detect Human CTACK by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. When used in conjunction with compatible development reagents the detection limit for recombinant Human CTACK is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Lagerung
-20 °C
Informationen zur Lagerung
The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-80°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.