IL-19 Antikörper (Biotin)

Produktnummer ABIN5690470

Der Kaninchen Polyklonal Anti-IL-19-Antikörper wurde für WB und ELISA validiert. Er ist geeignet, IL-19 in Proben von Human zu detektieren.

Kurzübersicht für IL-19 Antikörper (Biotin) (ABIN5690470)

Target: IL-19 (IL19) (Interleukin 19 (IL19))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IL-19 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-IL-19 Antikörper (Biotin)

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-19. Human IL-19 specific antibody was purified by affinity chromatography and then biotinylated.ced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-19. Human IL-19 specific antibody was purified by affinity chromatography employing immobilized hIL-19 matrix.

Anwendungsinformationen

Applikationshinweise: ELISA:
Direct:
To detect hIL-19 by direct ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-19.

Sandwich
To detect hIL-19 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human IL-19 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-19.

Western Blot:
To detect hIL-19 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-19 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Lagerung: -20 °C

Informationen zur Lagerung: IL-19 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Details zu IL-19

Target: IL-19 (IL19) (Interleukin 19 (IL19))

Andere Bezeichnung: IL-19

Hintergrund: IL19 is a cytokine that belongs to the IL10 cytokine subfamily. This cytokine is found to be preferentially expressed in monocytes. It can bind the IL20 receptor complex and lead to the activation of the signal transducer and activator of transcription 3 (STAT3). A similar cytokine in mouse is reported to up-regulate the expression of IL6 and TNF-alpha and induce apoptosis, which suggests a role of this cytokine in inflammatory responses. Little is known about the biologic function and gene regulation of IL19.

Gen-ID: 29949

UniProt: Q9UHD0