SMURF2 Antikörper (AA 140-190)

Produktnummer ABIN5690259

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch SMURF2 in WB, ELISA, IF und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte.

Kurzübersicht für SMURF2 Antikörper (AA 140-190) (ABIN5690259)

Target: SMURF2 (SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SMURF2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-SMURF2 Antikörper

Bindungsspezifität: AA 140-190

Spezifität: SMURF2 antibody is human, mouse and rat reactive. SMURF2 is predicted to not cross-react with SMURF1.

Aufreinigung: SMURF2 antibody is affinity chromatography purified via peptide column.

Immunogen: SMURF2 antibody was raised against a 17 amino acid peptide near the amino terminus of human SMURF2.

The immunogen is located within amino acids 140 - 190 of SMURF2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: SMURF2 antibody can be used for detection of SMURF2 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunohistochemistry at 2 μ,g/mL. For Immunoflorescence start at 20 μ,g/mL.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: SMURF2 Antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: SMURF2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Details zu SMURF2

Target: SMURF2 (SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2))

Andere Bezeichnung: SMURF2

Hintergrund: SMURF2 (SMAD ubiquitin regulatory factor 2) is a negative regulator of TGF-beta signaling (1). SMURF1 and SMURF2 are members of HECT domain E3 ubiquitin ligase which are involved in the enzymatic reactions of the Ub conjugating pathway (1,2). SMURF2 is widely expressed and was initially identified as an inhibitor of TGF-beta/BMP signaling by targeting R-Smads and TGF type I receptor for ubiquitination and degradation (3). Studies have shown that SMURF2 functions as a tumor suppressor by maintaining genomic stability through targeting RNF20 (3). SMURF2 associates constitutively with SMAD7 (4).

Molekulargewicht: Predicted: 82 kDa

Observed: 85 kDa

Gen-ID: 64750

NCBI Accession: NP_073576

UniProt: Q9HAU4