alpha Actinin 4 Antikörper (AA 27-38)

Produktnummer ABIN5689869

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch alpha Actinin 4 in WB, IHC und IP. Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Schwein und Hamster.

Kurzübersicht für alpha Actinin 4 Antikörper (AA 27-38) (ABIN5689869)

Target: alpha Actinin 4 (ACTN4) (Actinin, alpha 4 (ACTN4))

Reaktivität: Human, Maus, Ratte, Schwein, Hamster

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser alpha Actinin 4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Produktdetails anti-alpha Actinin 4 Antikörper

Bindungsspezifität: AA 27-38

Spezifität: Recognizes human, mouse, rat, pig and Chinese hamster actinin-4. Does not cross-react with actinin-1, -2 or -3.

Aufreinigung: Antigen affinity purified.

Immunogen: Synthetic peptide corresponding to aa 27-38 of the N-terminal alpha-Actinin-4, coupled via a C-terminal cysteine residue to KLH.

Anwendungsinformationen

Applikationshinweise: Immunocytochemistry: (1:250- 1:1,600) . Immunohistochemistry: (1:250- 1:1,600) . Immunoprecipitation: (1:10) . Western Blot: (1:1,000-1:2,500) . Optimal conditions must be determined individually for each application.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Liquid. In PBS containing 1 mg/mL BSA and 0.01 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for at least 1 year after receipt when stored at -20°C.

Details zu alpha Actinin 4

Target: alpha Actinin 4 (ACTN4) (Actinin, alpha 4 (ACTN4))

Andere Bezeichnung: alpha-Actinin-4

Hintergrund: Alpha-Actinin 4 is an actin-bundling protein of ~100  kDa that is associated with cell motility, endocytosis and cancer invasion. The alpha-actinin family comprises two non-muscle isoforms (alpha-actinin-1 and -4) and two skeletal muscle isoforms (alpha-actinin-2 and -3), with alpha-actinin-2 being also expressed in cardiac muscle. While alpha-actinin-4 is almost ubiquitously expressed, particularly high concentrations are found in glomeruli. On the subcellular level it is associated with actin stress fibers, but in certain cells it also localizes to the nucleus. Mutations in the alpha-actinin-4 gene cause an autosomal-dominant form of familial focal segmental glomerulosclerosis (FSGS), which is thought to result from a defect in glomerular podocyte function. A point mutation in the alpha-actinin-4 gene was found to generate an antigenic peptide that is recognized by autologous cytolytic T lymphocytes (CTL) on a human lung carcinoma. alpha-Actinin-4 interacts with a variety of proteins, including the ring finger protein BERP, the PDZ-LIM protein CLP-36, the hemidesmosomal and cell-cell contact protein BP180, and the tight junction protein MAGI-1. Moreover, alpha-actinin-4 forms a ternary complex with Ca2+/Calmodulin-dependent protein kinase II and densin-180, a protein of postsynaptic densities in CNS neurons. Ca2+-dependent association of alpha-actinin-4 with E3KARP is required for Ca2+-dependent inhibition of the Na+/H+ exchanger 3 (NHE3).

Gen-ID: 81

NCBI Accession: NP_004915

UniProt: O43707

Pathways: Proton Transport