PK (pThr446) Antikörper

Produktnummer ABIN5689715

Der Kaninchen Polyklonal anti-PK Antikörper (ABIN5689715) detektiert spezifisch PK in IF, IHC und WB. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für PK (pThr446) Antikörper (ABIN5689715)

Target: PK

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)

Produktdetails

Bindungsspezifität: pThr446

Spezifität: This antibody detects endogenous level of PKR only when phosphorylated at threonine 446.

Aufreinigung: Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Immunogen: PKR (Phospho-Thr446) antibody was raised against a peptide sequence around phosphorylation site of threonine 446 (K-R-T (p) -R-S) derived from Human PKR.

Anwendungsinformationen

Applikationshinweise: Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+),  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store antibody at -20°C for up to one year.

Antigendetails

Target: PK

Hintergrund: Following activation by double-stranded RNA in the presence of ATP, the kinase becomes autophosphorylated and can catalyze the phosphorylation of the translation initiation factor EIF2S1, which leads to an inhibition of the initiation of protein synthesis. Double-stranded RNA is generated during the course of a viral infection.

Molekulargewicht: 68 kDa

Gen-ID: 5610

NCBI Accession: NP_001129123

UniProt: P19525