Der Kaninchen-Polyklonal Anti-MARCKS-Antikörper wurde für WB und IF validiert. Er ist geeignet zum Nachweis von MARCKS in Proben aus Human, Maus und Ratte.
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This antibody detects endogenous level of MARCKS only when phosphorylated at serine 162.
Aufreinigung
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Immunogen
MARCKS (Phospho-Ser162) antibody was raised against a peptide sequence around phosphorylation site of serine 162 (K-K-S (p) -F-K) derived from Human MARCKS.
MARCKS
Reaktivität: Human
WB
Wirt: Kaninchen
Polyclonal
RB04646
unconjugated
Applikationshinweise
Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
1 mg/mL
Buffer
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
-20 °C
Informationen zur Lagerung
Store antibody at -20°C for up to one year.
Target
MARCKS
(Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS))
Andere Bezeichnung
MARCKS
Hintergrund
MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.