Tyrosine Hydroxylase Antikörper (AA 29-33)

Produktnummer ABIN5689619

Dieses Anti-Tyrosine Hydroxylase-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Tyrosine Hydroxylase in WB. Geeignet für Ratte und Maus.

Kurzübersicht für Tyrosine Hydroxylase Antikörper (AA 29-33) (ABIN5689619)

Target: Tyrosine Hydroxylase (TH)

Reaktivität: Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Tyrosine Hydroxylase Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-Tyrosine Hydroxylase Antikörper

Bindungsspezifität: AA 29-33

Spezifität: This antibody detects endogenous levels of total Tyrosine Hydroxylase protein.

Aufreinigung: Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Immunogen: Tyrosine Hydroxylase (Ab-31) antibody was raised against a peptide sequence around aa.29~33 (V-T-S-P-R) derived from Mouse Tyrosine Hydroxylase.

Anwendungsinformationen

Applikationshinweise: Western Blot: 1:500~1:1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+),  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store antibody at -20°C for up to one year.

Details zu Tyrosine Hydroxylase

Target: Tyrosine Hydroxylase (TH)

Andere Bezeichnung: Tyrosine Hydroxylase

Hintergrund: Tyrosine hydroxylase (TH) catalyzes the rate-limiting step in the synthesis of the neurotransmitter dopamine and other catecholamines. TH functions as a tetramer, with each subunit composed of a regulatory and catalytic domain, and exists in several different isoforms . This enzyme is required for embryonic development since TH knockout mice die before or at birth . Levels of transcription, translation and posttranslational modification regulate TH activity. The amino-terminal regulatory domain contains three serine residues: Ser9, Ser31 and Ser40. Phosphorylation at Ser40 by PKA positively regulates the catalytic activity of TH . Phosphorylation at Ser31 by CDK5 also increases the catalytic activity of TH through stabilization of TH protein levels.

Molekulargewicht: 55 - 60 kDa

Gen-ID: 7054

NCBI Accession: NP_954986

Pathways: Dopaminergic Neurogenesis, Response to Water Deprivation, Sensory Perception of Sound, Carbohydrate Homeostasis, Feeding Behaviour