CXCL2 Antikörper

Produktnummer ABIN5689185

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch CXCL2 in WB, ELISA und Neut. Er zeigt eine Reaktivität gegenüber Ratte.

Kurzübersicht für CXCL2 Antikörper (ABIN5689185)

Target: CXCL2 (Chemokine (C-X-C Motif) Ligand 2 (CXCL2))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CXCL2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Neutralization (Neut)

Produktdetails anti-CXCL2 Antikörper

Aufreinigung: Anti-Rat GROβ,/MIP-2 specific antibody was purified by affinity chromatography employing immobilized Rat GROβ,/MIP-2 matrix.

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant Rat GROβ,/MIP-2 (Rat GROβ,/Macrophage Inflammatory Protein-2).

Anwendungsinformationen

Applikationshinweise: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Rat GROβ,/MIP-2 (100 ng/mL), a concentration of 3.0 - 5.0 μ,g/mL of this antibody is required.

ELISA:

To detect Rat GROβ,/MIP-2 by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant Rat GROβ,/MIP-2.

Sandwich: To detect Rat GROβ,/MIP-2 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Rat GROβ,/MIP-2 (XP-5149Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat GROβ,/MIP-2.

Western Blot:

To detect Rat GROβ,/MIP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat GROβ,/MIP-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Lagerung: -20 °C

Informationen zur Lagerung: GROB/MIP-2 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Details zu CXCL2

Target: CXCL2 (Chemokine (C-X-C Motif) Ligand 2 (CXCL2))

Andere Bezeichnung: MIP-2

Hintergrund: Macrophage inflammatory protein 2 (MIP-2) is a chemokine that attracts neutrophils, and its secretion from intestinal epithelial cells is enhanced by inflammatory stimuli such as interleukin 1beta.

Gen-ID: 114105

UniProt: P30348

Pathways: Cellular Response to Molecule of Bacterial Origin