CCL25 Antikörper

Produktnummer ABIN5689117

Der Kaninchen Polyklonal Anti-CCL25-Antikörper wurde für ELISA, WB und Neut validiert. Er ist geeignet, CCL25 in Proben von Human zu detektieren.

Kurzübersicht für CCL25 Antikörper (ABIN5689117)

Target: CCL25 (Chemokine (C-C Motif) Ligand 25 (CCL25))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CCL25 Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Neutralization (Neut)

Produktdetails anti-CCL25 Antikörper

Aufreinigung: Anti-hTECK specific antibody was purified by affinity chromatography employing immobilized hTECK matrix.

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hTECK (human Thymus Expressed Chemokine).

Anwendungsinformationen

Applikationshinweise: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hTECK (100 ng/mL), a concentration of 10.0- 15.0 μ,g/mL of this antibody is required.

ELISA:

To detect hTECK by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hTECK.

Sandwich:

To detect hTECK by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human TECK (XP-5280Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTECK.

Western Blot:
To detect hTECK by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTECK is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Lagerung: -20 °C

Informationen zur Lagerung: TECK antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Details zu CCL25

Target: CCL25 (Chemokine (C-C Motif) Ligand 25 (CCL25))

Andere Bezeichnung: TECK

Hintergrund: This gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. TECK displays chemotactic activity for dendritic cells, thymocytes, and activated macrophages but is inactive on peripheral blood lymphocytes and neutrophils. TECK binds to chemokine receptor CCR9. Two alternatively spliced variants encoding two different isoforms have been identified.

Gen-ID: 6370

UniProt: O15444