Anti-hsRANK Receptor specific antibody was purified by affinity chromatography and then biotinylated.
Immunogen
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hsRANK Receptor (human sRANK Receptor).
Applikationshinweise
ELISA:
To detect hsRANK Receptor by direct ELISA (using 100 μ,L/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 μ,g/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant hsRANK Receptor.
Sandwich:
To detect hsRANK Receptor by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human sRANK Receptor (XP-5275) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hsRANK Receptor.
Western Blot:
To detect hsRANK Receptor by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsRANK Receptor is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Lagerung
-20 °C
Informationen zur Lagerung
sRank Receptor antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.