CXCL7 Antikörper

Produktnummer ABIN5689091

Der Kaninchen Polyklonal anti-CXCL7 Antikörper (ABIN5689091) detektiert spezifisch CXCL7 in WB, ELISA und Neut. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für CXCL7 Antikörper (ABIN5689091)

Target: CXCL7 (PPBP) (Pro-Platelet Basic Protein (Chemokine (C-X-C Motif) Ligand 7) (PPBP))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CXCL7 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Neutralization (Neut)

Produktdetails anti-CXCL7 Antikörper

Aufreinigung: Anti-hNAP-2 specific antibody was purified by affinity chromatography employing immobilized hNAP-2 matrix.

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hNAP-2 (human Neutrophil Activating Protein-2).

Anwendungsinformationen

Applikationshinweise: Neutralization:
To yield one-half maximal inhibition [ND50] oof the biological activity of hNAP-2 (50.00 ng/mL), a concentration of 1.75 - 3.0 μ,g/mL of this antibody is required.

ELISA:

To detect hNAP-2 by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hNAP-2.

Sandwich:

To detect hNAP-2 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human NAP-2 (XP-5244Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNAP-2.

Western Blot:
To detect hNAP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNAP-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Lagerung: -20 °C

Informationen zur Lagerung: NAP-2 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Details zu CXCL7

Target: CXCL7 (PPBP) (Pro-Platelet Basic Protein (Chemokine (C-X-C Motif) Ligand 7) (PPBP))

Andere Bezeichnung: NAP-2

Hintergrund: NAP2 is a platelet-derived growth factor that belongs to the CXC chemokine family. This growth factor is a potent chemoattractant and activator of neutrophils. It has been shown to stimulate various cellular processes including DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and sythesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by synovial cells.

Gen-ID: 5473

UniProt: P02775