Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hGRO-gamma (100 ng/mL), a concentration of 1.0 - 2.0 μ,g/mL of this antibody is required.
ELISA:
To detect hGRO-gamma by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hGRO-gamma.
Sandwich: To detect hGRO-γ, by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human GRO-γ, (XP-5150Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hGRO-γ,.
Western Blot: To detect hGRO-gamma by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGRO-gamma is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Lagerung
-20 °C
Informationen zur Lagerung
Gro-gamma antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.